Render Target: STATIC
Render Timestamp: 2024-11-01T10:30:28.281Z
Commit: 23cb9f61fe67e1e9093fd644a533c4ff516a6463
XML generation date: 2024-05-10 22:34:29.206
Product last modified at: 2024-10-24T10:45:19.857Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

Phospho-Tyrosine Mouse mAb (P-Tyr-100) #9411

Filter:
  • WB
  • IP
  • IHC
  • IF
  • F

    Supporting Data

    REACTIVITY All
    SENSITIVITY Endogenous
    MW (kDa)
    Source/Isotype Mouse IgG1
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    • IHC-Immunohistochemistry 
    • IF-Immunofluorescence 
    • F-Flow Cytometry 
    Species Cross-Reactivity Key:
    • All-All Species Expected 

    Product Information

    Product Usage Information

    The phosphorylated form of the peptide can be detected with Phospho-Tyrosine mAb (P-Tyr-100) #9411. Sample kinase protocol is attached.

    Application Dilution
    Western Blotting 1:2000
    Immunoprecipitation 1:100
    Immunohistochemistry (Paraffin) 1:2400 - 1:9600
    Immunofluorescence (Frozen) 1:1600 - 1:3200
    Immunofluorescence (Immunocytochemistry) 1:1600 - 1:3200
    Flow Cytometry (Fixed/Permeabilized) 1:1600 - 1:6400
    Peptide ELISA (DELFIA) 1:4000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    For a carrier free (BSA and azide free) version of this product see product #80379.

    Protocol

    Specificity / Sensitivity

    Phospho-Tyrosine Mouse mAb (P-Tyr-100) is a high affinity antibody. ELISAs against a wide variety of phosphopeptides indicate that P-Tyr-100 binds phospho-Tyr in a manner largely independent of the surrounding amino acid sequence. 2D gel Western blot analysis of pervanadate-treated cell extracts also shows that P-Tyr-100 interacts with a broad range of tyrosine-phosphorylated proteins. P-Tyr-100 does not cross-react with peptides containing phospho-Ser or phospho-Thr.

    Species Reactivity:

    All Species Expected

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with phospho-tyrosine containing peptides .

    Background

    Tyrosine phosphorylation plays a key role in cellular signaling (1). Research studies have shown that in cancer, unregulated tyrosine kinase activity can drive malignancy and tumor formation by generating inappropriate proliferation and survival signals (2). Antibodies specific for phospho-tyrosine (3,4) have been invaluable reagents in these studies. The phospho-tyrosine monoclonal antibodies developed by Cell Signaling Technology are exceptionally sensitive tools for studying tyrosine phosphorylation and monitoring tyrosine kinase activity in high throughput drug discovery.
    For Research Use Only. Not For Use In Diagnostic Procedures.
    Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
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