Phospho-WIPI2 (Ser413) Antibody #13571
- WB
- IP
Supporting Data
| REACTIVITY | H M R |
| SENSITIVITY | Endogenous |
| MW (kDa) | 49 |
| SOURCE | Rabbit |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:100 |
Storage
Protocol
Specificity / Sensitivity
Species Reactivity:
Source / Purification
Background
Vacuolar trafficking and autophagy are controlled by the class III type phosphoinositide 3-kinase (PI3K) Vps34, which generates phosphoinositide-3-phosphate (PtdIns3P) (4,5). Atg18 and Atg21 are two related WD-repeat proteins that bind PtdIns3P via a conserved Phe-Arg-Arg-Gly motif (6,7). It has been shown that Atg18 binds to Atg2 and that this complex is directed to vacuolar membranes by its interaction with PtdIns3P (8). Human orthologs of Atg18 and Atg21 were identified as members of the WD-repeat protein Interacting with Phosphoinositides (WIPI) family (9-11). WIPI1 (also called WIPI49) and WIPI2 have been shown to translocate from several vacuolar compartments to LC3-positive autophagosomes during autophagy; this translocation may be used as an autophagy marker (10).~
Phospho-WIPI2 (Ser413) Antibody is directed at a site that was identified at Cell Signaling Technology (CST) using PhosphoScan, CST's LC-MS/MS platform for modification site discovery. Phosphorylation at Ser413 was discovered using Phospho-MAPK/CDK Substrate motif antibody. Please visit PhosphoSitePlus, CST's modification site knowledgebase, at www.phosphosite.org for more information.
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- Yan, Y. and Backer, J.M. (2007) Biochem Soc Trans 35, 239-41.
- Krick, R. et al. (2006) FEBS Lett 580, 4632-8.
- Strømhaug, P.E. et al. (2004) Mol Biol Cell 15, 3553-66.
- Obara, K. et al. (2008) J Biol Chem 283, 23972-80.
- Jeffries, T.R. et al. (2004) Mol Biol Cell 15, 2652-63.
- Proikas-Cezanne, T. et al. (2007) FEBS Lett 581, 3396-404.
- Polson, H.E. et al. (2010) Autophagy 6, 506-22.
Limited Uses
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