Phospho-YAP (Ser127) (D9W2I) Rabbit mAb #13008
- WB
- IP
- IHC
Supporting Data
| REACTIVITY | H M R |
| SENSITIVITY | Endogenous |
| MW (kDa) | 65-78 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
- IHC-Immunohistochemistry
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:200 |
| Immunohistochemistry (Paraffin) | 1:625 - 1:2500 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
For a carrier free (BSA and azide free) version of this product see product #92367.
Protocol
Specificity / Sensitivity
Phospho-YAP (Ser127) (D9W2I) Rabbit mAb recognizes endogenous levels of YAP protein only when phosphorylated at Ser127. This antibody may cross-react with phospho-TAZ (Ser89).
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser127 of human YAP protein.
Background
YAP (Yes-associated protein, YAP65) was first identified based on its ability to associate with the SH3 domain of Yes. It also binds to other SH3 domain-containing proteins such as Nck, Crk, Src, and Abl (1). In addition to the SH3 binding motif, YAP contains a PDZ interaction motif, a coiled-coil domain, and WW domains (2-4). While initial studies of YAP all pointed towards a role in anchoring and targeting to specific subcellular compartments, subsequent studies showed that YAP is a transcriptional co-activator by virtue of its WW domain interacting with the PY motif (PPxY) of the transcription factor PEBP2 and other transcription factors (5). In its capacity as a transcriptional co-activator, YAP is now widely recognized as a central mediator of the Hippo Pathway, which plays a fundamental and widely conserved role in regulating tissue growth and organ size (6-8). Phosphorylation at multiple sites (e.g., Ser109, Ser127) by LATS kinases promotes YAP translocation from the nucleus to the cytoplasm, where it is sequestered through association with 14-3-3 proteins (7-9). These LATS-driven phosphorylation events serve to prime YAP for subsequent phosphorylation by CK1δ/ε in an adjacent phosphodegron, triggering proteasomal degradation of YAP (10).
- Sudol, M. (1994) Oncogene 9, 2145-52.
- Mohler, P.J. et al. (1999) J Cell Biol 147, 879-90.
- Espanel, X. and Sudol, M. (2001) J Biol Chem 276, 14514-23.
- Sudol, M. et al. (1995) FEBS Lett 369, 67-71.
- Yagi, R. et al. (1999) EMBO J 18, 2551-62.
- Dong, J. et al. (2007) Cell 130, 1120-33.
- Zhao, B. et al. (2010) Genes Dev 24, 862-74.
- Zhao, B. et al. (2007) Genes Dev 21, 2747-61.
- Yu, F.X. et al. (2012) Cell 150, 780-91.
- Zhao, B. et al. (2010) Genes Dev 24, 72-85.
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