Buy a SimpleChIP Kit and Try our New SimpleChIP® Universal qPCR Master Mix (88989P) for Free | Get The Code >>
3860
PLCγ Antibody Sampler Kit

PLCγ Antibody Sampler Kit #3860

Western Blotting Image 1

Western blot analysis of extracts from NIH/3T3 cells, untreated or PDGF-stimulated for the indicated times, using Phospho-PLCγ1 (Tyr783) Antibody (upper) or PLCγ1 Antibody #2822 (lower).

Learn more about how we get our images
Western Blotting Image 2

Western blot analysis of extracts from Ramos cells, untreated or treated with anti-human IgM, using Phospho-PLCgamma2 (Tyr1217) Antibody (upper) or PLCgamma2 Antibody #3872 (lower).

Learn more about how we get our images
Western Blotting Image 3

Western blot analysis of extracts from Ramos (lane 1), THP1 (lane 2), U-937 (lane 3) cells and mouse spleenocytes (lane 4), using PLCγ2 Antibody.

Learn more about how we get our images
Western Blotting Image 4

Western blot analysis of extracts from untreated or anti-human IgM treated Ramos cells or lamda phosphatase-treated Ramos cell lysates, using Phospho-PLCgamma2 (Tyr759) Antibody (upper) or PLCgamma2 Antibody #3872 (lower).

Learn more about how we get our images
Western Blotting Image 5

Western blot analysis of extracts from various cell lines using PLCγ1 (D9H10) XP® Rabbit mAb.

Learn more about how we get our images
Western Blotting Image 6

After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO® is added and emits light during enzyme catalyzed decomposition.

Learn more about how we get our images
Flow Cytometry Image 7

Flow cytometric analysis of NIH3T3 cells, untreated (blue) or PDGF treated (green), using Phospho-PLCγ1 (Tyr783) Antibody.

Learn more about how we get our images
Western Blotting Image 8

Western blot analysis of extracts from NIH/3T3 (lanes 1, 3, 5) and Ramos (lanes 2, 4, 6) cells, using PLCγ1 Antibody #2822 (lanes 1 and 2), PLCgamma2 Antibody (lanes 5 and 6) or both antibodies (lanes 3 and 4). Results show that PLCγ2 Antibody is specific to the 150 kDa PLCγ2 band detected in Ramos cells, while PLCγ1 Antibody #2822 is specifc to the 160 kDa PLCγ1 band detected in both Ramos and NIH/3T3 cells.

Learn more about how we get our images
Western Blotting Image 9

Western blot analysis of extracts from HeLa cells, either mock-transfected or transfected for 48 hours with SignalSilence™ PLCγ1 siRNA I #6293 or siRNA II #6254, using PLCγ1 (D9H10) XP® Rabbit mAb.

Learn more about how we get our images
IHC-P (paraffin) Image 10

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using PLCγ1 (D9H10) XP® Rabbit mAb.

Learn more about how we get our images
IHC-P (paraffin) Image 11

Immunohistochemical analysis of paraffin-embedded human lung carcioma using PLCγ1 (D9H10) XP® Rabbit mAb.

Learn more about how we get our images
Product Includes Quantity Applications Reactivity MW(kDa) Isotype
Phospho-PLCγ1 (Tyr783) Antibody 2821 20 µl
  • WB
  • F
H M R 155 Rabbit 
Phospho-PLCγ2 (Tyr1217) Antibody 3871 20 µl
  • WB
H M 150 Rabbit 
PLCγ2 Antibody 3872 20 µl
  • WB
  • IP
H M 150 Rabbit 
Phospho-PLCγ2 (Tyr759) Antibody 3874 20 µl
  • WB
H M 150 Rabbit 
PLCγ1 (D9H10) XP® Rabbit mAb 5690 20 µl
  • WB
  • IP
  • IHC
H M R Mk 150 Rabbit IgG
Anti-rabbit IgG, HRP-linked Antibody 7074 100 µl
  • WB
Goat 

PLCγ Antibody Sampler Kit provides an economical means of analyzing phospho and total PLCγ levels. PLCγ Antibody Sampler Kit contains enough primary and secondary antibodies to perform two western blot experiments with each antibody.

Each antibody in the PLCγ Antibody Sampler Kit detects endogenous levels of its target protein. The antibodies do not cross react with other PLCs.

PLCγ1 monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu1220 of human PLCγ1 protein.

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding human PLCγ1 and PLCγ2. Antibodies are purified by protein A and peptide affinity chromatography.

Phosphoinositide-specific phospholipase C (PLC) plays a significant role in transmembrane signaling. In response to extracellular stimuli such as hormones, growth factors, and neurotransmitters, PLC hydrolyzes phosphatidylinositol 4,5-bisphosphate (PIP2) to generate two secondary messengers: inositol 1,4,5-triphosphate (IP3) and diacylglycerol (DAG) (1). At least four families of PLCs have been identified: PLCβ, PLCγ, PLCδ, and PLCε. Phosphorylation is one of the key mechanisms that regulate the activity of PLC. PLCγ is activated by both receptor and non-receptor tyrosine kinases (2). PLCγ forms a complex with EGF and PDGF receptors, which leads to the phosphorylation of PLCγ at Tyr771, 783, and 1248 (3). Phosphorylation by Syk at Tyr783 activates the enzymatic activity of PLCγ1 (4). PLCγ2 is engaged in antigen-dependent signaling in B cells and collagen-dependent signaling in platelets. Phosphorylation by Btk or Lck at Tyr753, 759, 1197, and 1217 is correlated with PLCγ2 activity (5,6).

  1. Singer, W.D. et al. (1997) Annu Rev Biochem 66, 475-509.
  2. Margolis, B. et al. (1989) Cell 57, 1101-7.
  3. Kim, H.K. et al. (1991) Cell 65, 435-41.
  4. Wang, Z. et al. (1998) Mol Cell Biol 18, 590-7.
  5. Watanabe, D. et al. (2001) J Biol Chem 276, 38595-601.
  6. Ozdener, F. et al. (2002) Mol Pharmacol 62, 672-9.
Entrez-Gene Id
5335 , 5336
Swiss-Prot Acc.
P19174 , P16885
For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Upstream / Downstream

pathwayImage

Explore pathways related to this product.