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PhosphoSitePlus® Resource

  • Additional protein information
  • Analytical tools


Product Includes Quantity Applications Reactivity MW(kDa) Isotype
Phospho-PLCγ1 (Tyr783) Antibody 2821 20 µl
Western Blotting Flow Cytometry
H M R 155 Rabbit 
Phospho-PLCγ2 (Tyr1217) Antibody 3871 20 µl
Western Blotting
H M 150 Rabbit 
PLCγ2 Antibody 3872 20 µl
Western Blotting Immunoprecipitation
H M 150 Rabbit 
Phospho-PLCγ2 (Tyr759) Antibody 3874 20 µl
Western Blotting
H M 150 Rabbit 
PLCγ1 (D9H10) XP® Rabbit mAb 5690 20 µl
Western Blotting Immunoprecipitation Immunohistochemistry
H M R Mk 150 Rabbit IgG
Anti-rabbit IgG, HRP-linked Antibody 7074 100 µl
Western Blotting

Product Description

PLCγ Antibody Sampler Kit provides an economical means of analyzing phospho and total PLCγ levels. PLCγ Antibody Sampler Kit contains enough primary and secondary antibodies to perform two western blot experiments with each antibody.

Specificity / Sensitivity

Each antibody in the PLCγ Antibody Sampler Kit detects endogenous levels of its target protein. The antibodies do not cross react with other PLCs.

Source / Purification

PLCγ1 monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu1220 of human PLCγ1 protein.

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding human PLCγ1 and PLCγ2. Antibodies are purified by protein A and peptide affinity chromatography.

Phosphoinositide-specific phospholipase C (PLC) plays a significant role in transmembrane signaling. In response to extracellular stimuli such as hormones, growth factors, and neurotransmitters, PLC hydrolyzes phosphatidylinositol 4,5-bisphosphate (PIP2) to generate two secondary messengers: inositol 1,4,5-triphosphate (IP3) and diacylglycerol (DAG) (1). At least four families of PLCs have been identified: PLCβ, PLCγ, PLCδ, and PLCε. Phosphorylation is one of the key mechanisms that regulate the activity of PLC. PLCγ is activated by both receptor and non-receptor tyrosine kinases (2). PLCγ forms a complex with EGF and PDGF receptors, which leads to the phosphorylation of PLCγ at Tyr771, 783, and 1248 (3). Phosphorylation by Syk at Tyr783 activates the enzymatic activity of PLCγ1 (4). PLCγ2 is engaged in antigen-dependent signaling in B cells and collagen-dependent signaling in platelets. Phosphorylation by Btk or Lck at Tyr753, 759, 1197, and 1217 is correlated with PLCγ2 activity (5,6).

1.  Singer, W.D. et al. (1997) Annu Rev Biochem 66, 475-509.

2.  Margolis, B. et al. (1989) Cell 57, 1101-7.

3.  Kim, H.K. et al. (1991) Cell 65, 435-41.

4.  Wang, Z. et al. (1998) Mol Cell Biol 18, 590-7.

5.  Watanabe, D. et al. (2001) J Biol Chem 276, 38595-601.

6.  Ozdener, F. et al. (2002) Mol Pharmacol 62, 672-9.

Entrez-Gene Id 5335 , 5336
Swiss-Prot Acc. P19174 , P16885

For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

PLCγ Antibody Sampler Kit