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9094
StemLight™ Pluripotency Surface Marker Antibody Kit
Primary Antibodies
Antibody Sampler Kit

StemLight™ Pluripotency Surface Marker Antibody Kit #9094

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StemLight™ Pluripotency Surface Marker Antibody Kit: Image 1

Confocal immunofluorescent analysis of NTERA-2 cells, using TRA-1-60(S) (TRA-1-60(S)) Mouse mAb, TRA-1-81 (TRA-1-81) Mouse mAb, and SSEA4 (MC813) Mouse mAb. Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

StemLight™ Pluripotency Surface Marker Antibody Kit: Image 2

Flow cytometric analysis of Jurkat cells (blue) and NTERA-2 cells (green) using SSEA4 (MC813) Mouse mAb (solid lines) or a concentration-matched Mouse (G3A1) mAb IgG1 Isotype Control #5415 (dashed lines). Anti-mouse IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4408 was used as a secondary antibody.

StemLight™ Pluripotency Surface Marker Antibody Kit: Image 3

Flow cytometric analysis of unpermeabilized Jurkat cells (blue) and unpermeabilized NTERA2 cells (green) using TRA-1-60(S) (TRA-1-60(S)) Mouse mAb.

StemLight™ Pluripotency Surface Marker Antibody Kit: Image 4

Flow cytometric analysis of unpermeabilized Jurkat cells (blue) and unpermeabilized NCCIT cells (green) using TRA-1-81 (TRA-1-81) Mouse mAb.

StemLight™ Pluripotency Surface Marker Antibody Kit: Image 5

Confocal immunofluorescent analysis of NTERA2 cells using SSEA4 (MC813) Mouse mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).

StemLight™ Pluripotency Surface Marker Antibody Kit: Image 6

Confocal immunofluorescent analysis of nTERA2 cells using TRA-1-60(S) (TRA-1-60(S)) Mouse mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

StemLight™ Pluripotency Surface Marker Antibody Kit: Image 7

Confocal immunofluorescent analysis of NTERA-2 cells using TRA-1-81 (TRA-1-81) Mouse mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

StemLight™ Pluripotency Surface Marker Antibody Kit: Image 8

Immunohistochemical analysis of paraffin-embedded NTERA (positive, left) and Jurkat (negative, right) cell pellets using TRA-1-60(S) (TRA-1-60(S)) Mouse mAb.

StemLight™ Pluripotency Surface Marker Antibody Kit: Image 9

Immunohistochemical analysis of paraffin-embedded NTERA (positive, left) and Jurkat (negative, right) cell pellets using TRA-1-81 (TRA-1-81) Mouse mAb.

StemLight™ Pluripotency Surface Marker Antibody Kit: Image 10

Western blot analysis of extracts from various cell lines using TRA-1-60 (TRA-1-60) Mouse mAb.

To Purchase # 9094T
Product # Size Price
9094T
1 Kit  (3 x 20 µl) $ 261

Product Includes Quantity Applications Reactivity MW(kDa) Isotype
SSEA4 (MC813) Mouse mAb 4755 20 µl
  • IF
  • F
H N/A Mouse IgG3
TRA-1-60(S) (TRA-1-60(S)) Mouse mAb 4746 20 µl
  • WB
  • IHC
  • IF
  • F
H 200-400 Mouse IgM
TRA-1-81 (TRA-1-81) Mouse mAb 4745 20 µl
  • IHC
  • IF
  • F
H 200-400 Mouse IgM

Product Description

The StemLight™ Surface Marker Kit contains a panel of antibodies for the detection of antigens that are specifically expressed on the surface of human pluripotent cells. The kit can be used to track the pluripotent potential of human embryonic stem (ES) or induced pluripotent (iPS) cells. The loss of these markers indicates a loss of pluripotency or differentiation of the culture. The kit components are pre-optimized for parallel use in immunofluorescent analysis.

Specificity / Sensitivity

TRA-1-60(S) (TRA-1-60(S)) Mouse mAb detects endogenous levels of the neuraminidase-sensitive TRA-1-60 antigen. TRA-1-81 (TRA-1-81) Mouse mAb detects endogenous levels of TRA-1-81 antigen. SSEA4 (MC813) Mouse mAb detects endogenous levels of SSEA4 antigen.

Source / Purification

Monoclonal antibodies are produced by immunizing animals with human embryonic carcinoma 2102Ep cl.2A6 cells.

Background

Pluripotency is the ability of a cell to differentiate into cell types of the three germ layers, the endoderm, ectoderm, and mesoderm. It is a property shared by embryonic stem cells, embryonic carcinoma, and induced pluripotent cells.

SSEA4, TRA-1-81, and TRA-1-60 antibodies recognize antigens expressed on the cell surface of all pluripotent cells. SSEA4 recognizes a glycolipid carbohydrate epitope (1). TRA-1-60(S) and TRA-1-81 antibodies recognize different proteoglycan epitopes on variants of the same protein, podocalyxin (2). These epitopes are neuraminidase sensitive and resistant, respectively. Reactivity of SSEA4, TRA-1-81 and TRA-1-60 antibodies with their respective cell surface markers are lost upon differentiation of pluripotent cells, corresponding with a loss of pluripotent potential (3).

  1. Henderson, J.K. et al. (2002) Stem Cells 20, 329-37.
  2. Draper, J.S. et al. (2002) J Anat 200, 249-58.
  3. Schopperle, W.M. and DeWolf, W.C. (2007) Stem Cells 25, 723-30.

Pathways & Proteins

Explore pathways + proteins related to this product.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST's products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST's Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
StemLight™ is a trademark of Cell Signaling Technology, Inc.
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