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To Purchase # 9094T
| 9094T | 1 Kit (3 x 20 µl) | $239.00 |
| $0.00 | ||
StemLight™ Pluripotency Surface Marker Antibody Kit #9094
Confocal immunofluorescent analysis of NTERA-2 cells, using TRA-1-60(S) (TRA-1-60(S)) Mouse mAb, TRA-1-81 (TRA-1-81) Mouse mAb, and SSEA4 (MC813) Mouse mAb. Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
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Flow cytometric analysis of unpermeabilized Jurkat cells (blue) and unpermeabilized NTERA cells (green) using SSEA4 (MC813) Mouse mAb.
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Western blot analysis of extracts from various cell lines using TRA-1-60 (TRA-1-60) Mouse mAb.
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Immunohistochemical analysis of paraffin-embedded NTERA (positive, left) and Jurkat (negative, right) cell pellets using TRA-1-81 (TRA-1-81) Mouse mAb.
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Confocal immunofluorescent analysis of NTERA2 cells using SSEA4 (MC813) Mouse mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).
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Immunohistochemical analysis of paraffin-embedded NTERA (positive, left) and Jurkat (negative, right) cell pellets using TRA-1-60(S) (TRA-1-60(S)) Mouse mAb.
Learn more about how we get our images
Flow cytometric analysis of unpermeabilized Jurkat cells (blue) and unpermeabilized NCCIT cells (green) using TRA-1-81 (TRA-1-81) Mouse mAb.
Learn more about how we get our images
Flow cytometric analysis of unpermeabilized Jurkat cells (blue) and unpermeabilized NTERA2 cells (green) using TRA-1-60(S) (TRA-1-60(S)) Mouse mAb.
Learn more about how we get our images
Confocal immunofluorescent analysis of NTERA-2 cells using TRA-1-81 (TRA-1-81) Mouse mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
Learn more about how we get our images
Confocal immunofluorescent analysis of nTERA2 cells using TRA-1-60(S) (TRA-1-60(S)) Mouse mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
Learn more about how we get our images
| Product Includes | Quantity | Applications | Reactivity | MW(kDa) | Isotype |
|---|---|---|---|---|---|
| SSEA4 (MC813) Mouse mAb 4755 | 20 µl |
|
H | N/A | Mouse IgG3 |
| TRA-1-60(S) (TRA-1-60(S)) Mouse mAb 4746 | 20 µl |
|
H | 200-400 | Mouse IgM |
| TRA-1-81 (TRA-1-81) Mouse mAb 4745 | 20 µl |
|
H | 200-400 | Mouse IgM |
Product Usage Information
Protocols
Product Description
The StemLight™ Surface Marker Kit contains a panel of antibodies for the detection of antigens that are specifically expressed on the surface of human pluripotent cells. The kit can be used to track the pluripotent potential of human embryonic stem (ES) or induced pluripotent (iPS) cells. The loss of these markers indicates a loss of pluripotency or differentiation of the culture. The kit components are pre-optimized for parallel use in immunofluorescent analysis.
Specificity / Sensitivity
TRA-1-60(S) (TRA-1-60(S)) Mouse mAb detects endogenous levels of the neuraminidase-sensitive TRA-1-60 antigen. TRA-1-81 (TRA-1-81) Mouse mAb detects endogenous levels of TRA-1-81 antigen. SSEA4 (MC813) Mouse mAb detects endogenous levels of SSEA4 antigen.
Source / Purification
Monoclonal antibodies are produced by immunizing animals with human embryonic carcinoma 2102Ep cl.2A6 cells.
Background
Pluripotency is the ability of a cell to differentiate into cell types of the three germ layers, the endoderm, ectoderm, and mesoderm. It is a property shared by embryonic stem cells, embryonic carcinoma, and induced pluripotent cells.
SSEA4, TRA-1-81, and TRA-1-60 antibodies recognize antigens expressed on the cell surface of all pluripotent cells. SSEA4 recognizes a glycolipid carbohydrate epitope (1). TRA-1-60(S) and TRA-1-81 antibodies recognize different proteoglycan epitopes on variants of the same protein, podocalyxin (2). These epitopes are neuraminidase sensitive and resistant, respectively. Reactivity of SSEA4, TRA-1-81 and TRA-1-60 antibodies with their respective cell surface markers are lost upon differentiation of pluripotent cells, corresponding with a loss of pluripotent potential (3).
1. Henderson, J.K. et al. (2002) Stem Cells 20, 329-37.
2. Draper, J.S. et al. (2002) J Anat 200, 249-58.
3. Schopperle, W.M. and DeWolf, W.C. (2007) Stem Cells 25, 723-30.
Data Sheets & Documentation
For Research Use Only. Not For Use In Diagnostic Procedures. Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc. StemLight™ is a trademark of Cell Signaling Technology, Inc. DRAQ5 is a registered trademark of Biostatus Limited.
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