Render Target: STATIC
Render Timestamp:
3/25/2025, 6:32:58 AM EDT
3/25/2025, 10:32:58 AM UTC
Commit: 8d93f7ebe45006d66c127727d817fc3f57c4fe9a
XML generation date: 2025-03-07 13:16:32.856
Product last modified at: 2025-02-26T14:30:10.829Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
  1. Products /
  2. Primary Antibodies /
  3. PLVAP (E9W8D) Rabbit mAb
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

PLVAP (E9W8D) Rabbit mAb #37176

Filter:
  • WB
  • IP
  • IF

    Supporting Data

    REACTIVITY M
    SENSITIVITY Endogenous
    MW (kDa) 62, 140
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    • IF-Immunofluorescence 
    Species Cross-Reactivity Key:
    • M-Mouse 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:200
    Immunofluorescence (Frozen) 1:200 - 1:800
    Immunofluorescence (Immunocytochemistry) 1:200 - 1:800

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    PLVAP (E9W8D) Rabbit mAb recognizes endogenous levels of total PLVAP protein. PLVAP exists as monomers and dimers under denatured and reducing conditions on Western blot and this antibody preferably recognizes the dimers.

    Species Reactivity:

    Mouse

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with recombinant mouse PLVAP protein.

    Background

    PLVAP (plasmalemma vesicle-associated protein) is an endothelial cell (EC)-specific type II membrane protein. It forms diaphragms that bridge the opening of caveolae, fenestrae, and trans-endothelial channels and plays a role in regulating vascular permeability. PLVAP expression is restricted to a subset of ECs in the normal microvasculature, but it is not expressed in the ECs of large vessels, with the exception of the endocardial lining of the heart chambers (1). While PLVAP is expressed in ECs in the brain and retina during embryogenesis and postnatal development, it is not expressed in mature endothelium that forms blood–brain barrier (BBB) and blood–retinal barrier (BRB). However, under pathological conditions associated with barrier disruption such as brain ischemia, cancer, and diabetic retinopathy, PLVAP expression is induced in endothelium of BBB and BRB (2). PLVAP was previously considered as an EC marker of the vascular system, its expression is now reported in lymphatic ECs (LECs) in lymph nodes and it may play a role in regulating the parenchymal entry of lymphocytes and soluble antigens (3,4).

    Database Links

    UniProt ID: Q91VC4


    Entrez-Gene Id: 84094


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