Cat. # | Size | Qty. | Price |
---|---|---|---|
67751S | 100 µl |
|
REACTIVITY | H |
SENSITIVITY | Endogenous |
MW (kDa) | 15 |
Source/Isotype | Rabbit IgG |
Product Information
Application | Dilution |
---|---|
Western Blotting | 1:1000 |
For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
NOTE: Please refer to primary antibody product webpage for recommended antibody dilution.
From sample preparation to detection, the reagents you need for your Western Blot are now in one convenient kit: #12957 Western Blotting Application Solutions Kit
NOTE: Prepare solutions with reverse osmosis deionized (RODI) or equivalent grade water.
Load 20 µl onto SDS-PAGE gel (10 cm x 10 cm).
NOTE: Loading of prestained molecular weight markers (#59329, 10 µl/lane) to verify electrotransfer and biotinylated protein ladder (#7727, 10 µl/lane) to determine molecular weights are recommended.
NOTE: Volumes are for 10 cm x 10 cm (100 cm2) of membrane; for different sized membranes, adjust volumes accordingly.
* Avoid repeated exposure to skin.
posted June 2005
revised June 2020
Protocol Id: 10
Human
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human PMP2 protein.
Myelin sheaths are multi-layered membranes derived from oligodendrocytes that increase the conduction velocity of axonal impulses. Peripheral myelin protein 2 (PMP2) is a small, 14 kDa cytoplasmic myelin protein expressed by Schwann cells in the periphery of vertebrates, as well as astrocytes and oligodendrocytes in humans. A member of the fatty acid binding protein (FABP) family, PMP2 is characterized by its folded β-barrel structure and α-helical cap. This structure allows PMP2 to bind lipid bilayers on two opposing faces, sticking membranes together with even spacing. PMP2 has been suggested to play roles in lipid homeostasis, lipid transport, and the remyelination of peripheral nerves post-injury. Mutations in the PMP2 gene are associated with Charcot-Marie-Tooth disease Type 1 (CMT1), the most common class of the heterogeneous group of CMT motor and sensory neuropathies. CMT1 is characterized by the loss of myelin and reduction of conduction velocity. Individuals with CMT1 suffer from sensory loss, muscle atrophy, and foot deformity (1-3).
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