Cell Signaling Technology Logo - Extra Large

PP1 alpha Antibody #2582

Filter:
  • WB

    Product Specifications

    REACTIVITY H M R Mk Dm
    SENSITIVITY Endogenous
    MW (kDa) 38
    SOURCE Rabbit
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 
    • Mk-Monkey 
    • Dm-D. melanogaster 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    PP1 alpha Antibody detects endogenous levels of total PP1α protein. This antibody detects recombinant levels of PP1γ and does not cross-react with PP1δ.

    Species Reactivity:

    Human, Mouse, Rat, Monkey, D. melanogaster

    Source / Purification

    Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the amino-terminal sequence of human PP1alpha. Antibodies are purified by protein A and peptide affinity chromatography.

    Background

    Type 1 protein phosphatase (PP1), a serine/threonine phosphatase, is highly conserved in eukaryotic cells. Four isoforms of PP1 have been characterized: PP1α, PP1δ, PP1γ1 and PP1γ2 (1). Involvement in cell cycle regulation is one of the biological functions of PP1 (1). It has been illustrated that PP1 dephosphorylates Rb and cdc25 during mitosis (2,3). A cell cycle-dependent phosphorylation at Thr320 of PP1α by cdc2 kinase inhibits PP1α activity (4).

    Alternate Names

    MGC15877; MGC1674; PP-1A; PP1A; PP1alpha; PPP1A; PPP1CA; protein phosphatase 1 catalytic subunit alpha; protein phosphatase 1, catalytic subunit, alpha isoform; protein phosphatase 1, catalytic subunit, alpha isozyme; serine/threonine protein phosphatase PP1-alpha 1 catalytic subunit; Serine/threonine-protein phosphatase PP1-alpha catalytic subunit; testicular tissue protein Li 155

    For Research Use Only. Not for Use in Diagnostic Procedures.
    Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
    All other trademarks are the property of their respective owners. Visit our Trademark Information page.