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64815
PhosphoPlus® PRAS40 (Thr246) Antibody Duet

PhosphoPlus® PRAS40 (Thr246) Antibody Duet #64815

Western Blotting Image 1

Western blot analysis of extracts from serum starved H3255, Mkn45 and NIH/3T3 cells, untreated or treated with either Gefitinib (1 μM, 3 hours), Su11274 (1 μM, 3 hours) or insulin (150 nM, 15 minutes), using Phospho-PRAS40 (Thr246) (C77D7) Rabbit mAb (upper) or PRAS40 (D23C7) Rabbit mAb #2691 (lower).

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Western Blotting Image 2

Western blot analysis of extracts from various cell types using PRAS40 (D23C7) XP® Rabbit mAb.

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Western Blotting Image 3

Western blot analysis of extracts from serum starved HeLa cells, untreated or treated with insulin (100 nM, 5 minutes) or with insulin and λ phosphatase, using Phospho-PRAS40 (Thr246) (C77D7) Rabbit mAb (upper) or PRAS40 Antibody #2610 (lower).

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IHC-P (paraffin) Image 4

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using PRAS40 (D23C7) XP® Rabbit mAb.

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IHC-P (paraffin) Image 5

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Phospho-PRAS40 (Thr246) (C77D7) Rabbit mAb in the presence of control peptide (left) or antigen specific peptide (right).

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IHC-P (paraffin) Image 6

Immunohistochemical analysis of paraffin-embedded human breast carcinoma, control (left) or λ phosphatase-treated (right), using Phospho-PRAS40 (Thr246) (C77D7) Rabbit mAb.

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IHC-P (paraffin) Image 7

Immunohistochemical analysis of paraffin-embedded metastatic SKOV-3 tumor in mouse lung using Phospho-PRAS40 (Thr246) (C77D7) Rabbit mAb.

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Product Includes Quantity Applications Reactivity MW(kDa) Isotype
Phospho-PRAS40 (Thr246) (C77D7) Rabbit mAb 2997 100 µl
  • WB
  • IP
  • IHC
H M R Mk 40 Rabbit IgG
PRAS40 (D23C7) XP® Rabbit mAb 2691 100 µl
  • WB
  • IP
  • IHC
H M R Mk 40 Rabbit IgG

PhosphoPlus® Duets from Cell Signaling Technology (CST) provide a means to assess protein activation status. Each Duet contains an activation-state and total protein antibody to your target of interest. These antibodies have been selected from CST's product offering based upon superior performance in specified applications.

Many growth factors and hormones induce the phosphoinositide 3-kinase signaling pathway, which results in the activation of downstream effector proteins such as the serine/threonine kinase Akt (1,2). One known Akt substrate is a 40 kDa, proline-rich protein (PRAS40) that binds to 14-3-3 proteins (2). PRAS40 also binds mTOR to transduce Akt signals to the mTOR complex. Inhibition of mTOR signaling stimulates PRAS40 binding to mTOR, which in turn inhibits mTOR activity (3). PRAS40 interacts with raptor in mTOR complex 1 (mTORC1) in insulin-deprived cells and inhibits the activation of the mTORC1 pathway mediated by the cell cycle protein Rheb. Phosphorylation of PRAS40 by Akt at Thr246 relieves PRAS40 inhibition of mTORC1 (4). mTORC1 in turn phosphorylates PRAS40 at Ser183 (5).

  1. Cantley, L.C. (2002) Science 296, 1655-7.
  2. Kovacina, K.S. et al. (2003) J Biol Chem 278, 10189-94.
  3. Vander Haar, E. et al. (2007) Nat Cell Biol 9, 316-23.
  4. Sancak, Y. et al. (2007) Mol Cell 25, 903-15.
  5. Oshiro, N. et al. (2007) J Biol Chem 282, 20329-39.
Entrez-Gene Id
84335
Swiss-Prot Acc.
Q96B36
For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
CST is a trademark of Cell Signaling Technology, Inc.
PhosphoPlus is a trademark of Cell Signaling Technology, Inc.
XP is a registered trademark of Cell Signaling Technology, Inc.
U.S. Patent No. 7,429,487, foreign equivalents, and child patents deriving therefrom.

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