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PRK2 Antibody #2612

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  • WB
  • IP

    Product Specifications

    REACTIVITY H M R
    SENSITIVITY Endogenous
    MW (kDa) 140
    SOURCE Rabbit
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:50

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    PRK2 Antibody detects endogenous levels of total PRK2 protein. This antibody does not cross-react with PRK1.

    Species Reactivity:

    Human, Mouse, Rat

    Source / Purification

    Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the amino-terminal sequence of human PRK2. Antibodies are purified by protein A and peptide affinity chromatography.

    Background

    The protein kinase C-related kinases (PRKs) are a subfamily of Ser/Thr-specific kinases with a catalytic domain highly homologous to the PKC family (1-3). They are effectors of Rho family GTPases (4-6) and are activated by fatty acids and phospholipids in vitro (7,8). Activation in vitro and in vivo involves the activation loop phosphorylation of PRK1 (Thr774)/PRK2 (Thr816) by PDK1 (9,10).

    Alternate Names

    cardiolipin-activated protein kinase Pak2; MGC150606; MGC71074; Pak-2; PAK2; PKN gamma; PKN2; PRK2; PRKCL2; PRO2042; Protein kinase C-like 2; protein kinase N2; Protein-kinase C-related kinase 2; Serine/threonine-protein kinase N2; STK7

    For Research Use Only. Not for Use in Diagnostic Procedures.
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