PRMT5 Antibody #2252
- WB
Product Specifications
| REACTIVITY | H M R Mk |
| SENSITIVITY | Endogenous |
| MW (kDa) | 73 |
| SOURCE | Rabbit |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
- Mk-Monkey
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Protocol
Specificity / Sensitivity
Species Reactivity:
Source / Purification
Background
PRMT5 is the predominant Type II PRMT and was first identified through interaction with Jak2 (4). It can catalyze the symmetric di-methylation of histone H2A and H4 on arginine 3 and histone H3 on arginine 2 and 8 (5-6). Methylation of H2A and H4 is thought to be inactivating through the recruitment of DNA methyltransferases, while methylation of H3 recruits WDR5 and MLL, thus promoting the maintenance of euchromatin (5,7). Other putative roles for PRMT5 have been shown in development, mRNA splicing, and chromatin remodeling (8-10). PRMT5 has been shown to be overexpressed in many different types of cancers (11-14).
- Bedford, M.T. and Richard, S. (2005) Mol Cell 18, 263-72.
- Wang, Y. et al. (2004) Science 306, 279-83.
- Cheng, D. et al. (2007) Mol Cell 25, 71-83.
- Pollack, B.P. et al. (1999) J Biol Chem 274, 31531-42.
- Migliori, V. et al. (2012) Nat Struct Mol Biol 19, 136-44.
- Branscombe, T.L. et al. (2001) J Biol Chem 276, 32971-6.
- Tsutsui, T. et al. (2013) J Biol Chem 288, 20955-65.
- Tee, W.W. et al. (2010) Genes Dev 24, 2772-7.
- Gonsalvez, G.B. et al. (2007) J Cell Biol 178, 733-40.
- Dacwag, C.S. et al. (2007) Mol Cell Biol 27, 384-94.
- Wang, L. et al. (2008) Mol Cell Biol 28, 6262-77.
- Bao, X. et al. (2013) J Histochem Cytochem 61, 206-17.
- Nicholas, C. et al. (2013) PLoS One 8, e74710.
- Gu, Z. et al. (2012) Biochem J 446, 235-41.
Alternate Names
72 kDa ICln-binding protein; ANM5; Histone-arginine N-methyltransferase PRMT5; HMT1 hnRNP methyltransferase-like 5; HRMT1L5; HSL7; IBP72; Jak-binding protein 1; JBP1; PRMT5; protein arginine methyltransferase 5; Protein arginine N-methyltransferase 5; Protein arginine N-methyltransferase 5, N-terminally processed; Shk1 kinase-binding protein 1 homolog; SKB1; SKB1 homolog; SKB1Hs
Limited Uses
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