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  1. Products /
  2. Primary Antibodies /
  3. Procathepsin L (E9B2U) Rabbit mAb
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Recombinant Flag
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

Procathepsin L (E9B2U) Rabbit mAb #84149

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  • WB

    Supporting Data

    REACTIVITY H Mk
    SENSITIVITY Endogenous
    MW (kDa) 42
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 
    • Mk-Monkey 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Simple Western™ 1:10 - 1:50

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    Procathepsin L (E9B2U) Rabbit mAb recognizes endogenous levels of total procathepsin L protein. This antibody detects the procathepsin L proprotein, but does not detect the cleaved (proteolytically active) form of cathepsin L protein.

    Species Reactivity:

    Human, Monkey

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu110 of human procathepsin L protein.

    Background

    Cathepsin L belongs to the C1 family of peptidases, a large and diverse family of cysteine (thiol) proteases that play fundamental roles in protein processing and degradation. The catalytic activity of C1 peptidases maps to a nucleophilic cysteine thiol, usually found within a catalytic dyad or triad (1). Cathepsin L, initially synthesized as procathepsin L before undergoing proteolytic cleavage to its active form, is a ubiquitously expressed thiol protease, expressed primarily in lysosomes but which is also secreted by some cell types. Its diverse functions include general protein catabolism and autophagy (2), antigen processing (3), promoting angiogenesis (4), and extracellular matrix degradation (5,6). An isoform of cathepsin L has also been reported in the nucleus, where it was shown to be involved in the regulation of transcription factor activity (7). Targeted deletion of the Ctsl gene in mice embryos was non-lethal, but resulted in periodic hair loss and skin defects, including epidermal hyperplasia, acanthosis, and hyperkeratosis (8). More recently, research studies have reported that cathepsin L is involved in viral protein processing during viral replication, and that cathepsin L inhibitors may have utility in combating infection by SARS-CoV-2 and related coronaviruses (9).

    Database Links

    UniProt ID: P07711


    Entrez-Gene Id: 1514


    Limited Uses

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    For Research Use Only. Not For Use In Diagnostic Procedures.
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