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  1. Products /
  2. Primary Antibodies /
  3. PSPC1 (E7L8B) Rabbit mAb
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Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

PSPC1 (E7L8B) Rabbit mAb #65992

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  • WB
  • IF

    Supporting Data

    REACTIVITY H M
    SENSITIVITY Endogenous
    MW (kDa) 70
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IF-Immunofluorescence 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunofluorescence (Immunocytochemistry) 1:100 - 1:400

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    PSPC1 (E7L8B) Rabbit mAb recognizes endogenous levels of total PSPC1 protein. Faint bands running around 30 kDa, 80 kDa, and 100 kDa were observed. We are unclear of the identity of these bands.

    Species Reactivity:

    Human, Mouse

    The antigen sequence used to produce this antibody shares 100% sequence homology with the species listed here, but reactivity has not been tested or confirmed to work by CST. Use of this product with these species is not covered under our Product Performance Guarantee.

    Species predicted to react based on 100% sequence homology:

    Rat

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human PSPC1 protein.

    Background

    PSPC1 (paraspeckle component 1) was one of the first proteins found to be enriched in subnuclear bodies that are adjacent to but distinct from nuclear speckles, and subsequently named paraspeckles (1). PSPC1 belongs to the Drosophila melanogaster behavior protein family comprised of human splicing (DBHS) proteins, including SFPQ (splicing factor proline-glutamine rich ) and NONO (non-POU domain-containing octamer-binding protein) which are also enriched in paraspeckles (2). DBHS proteins are a unique family of RNA-binding proteins that recruit various proteins and nucleic acids involved in transcriptional activation and repression, transcript localization, splicing, and DNA damage repair (3). Important to disease, DBHS proteins bind the long noncoding RNA NEAT1 which is found to be enriched in paraspeckles. Aberrant accumulation of NEAT1 transcripts in paraspeckles has been identified in multiple tumor types, leading to a proposal that the long coding RNA, via the process of sponging, disrupts the ratio of miRNAs to target proteins, resulting in altered protein expression profiles that promote cancer (4).

    Database Links

    UniProt ID: Q8WXF1


    Entrez-Gene Id: 55269


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    For Research Use Only. Not For Use In Diagnostic Procedures.
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