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Render Timestamp: 2024-12-05T11:42:57.409Z
Commit: 224419269841c11382c4555dbee545259bf6c379
XML generation date: 2024-07-30 10:05:11.841
Product last modified at: 2024-11-20T17:15:08.061Z
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PDP - Template Name: Antibody Sampler Kit
PDP - Template ID: *******4a3ef3a

PTEN and PDK1 Antibody Sampler Kit II #30590

    Product Information

    Product Description

    The PTEN and PDK1 Antibody Sampler Kit II provides an economical means to evaluate two key enzymes that regulate multiple signaling pathways. The kit includes enough antibodies to perform two western blot experiments with each primary antibody.

    Specificity / Sensitivity

    PTEN (D4.3) XP® Rabbit mAb detects endogenous levels of total PTEN protein. Phospho-PTEN (Ser380/Thr382/383) (44A7) Rabbit mAb detects endogenous levels of PTEN when phosphorylated at Ser380, Thr382 and Thr383. It may also react with PTEN singly phosphorylated at Ser380. PDK1 (D37A7) Rabbit mAb detects endogenous levels of total PDK1 protein. Phospho-PDK1 (Ser241) (C49H2) Rabbit mAb detects PDK1 only when phosphorylated at Ser241.

    Source / Purification

    PTEN (D4.3) XP® Rabbit mAb is produced by immunizing animals with a synthetic peptide corresponding to residues in the carboxy-terminal sequence of human PTEN. Phospho-PTEN (Ser380/Thr382/383) (44A7) Rabbit mAb is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues around Ser380, Thr382 and Thr383 of human PTEN. PDK1 (D37A7) Rabbit mAb is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human PDK1 protein. Phospho-PDK1 (Ser241) (C49H2) Rabbit mAb is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues around Ser241 of human PDK1.

    Background

    PTEN (phosphatase and tensin homologue deleted on chromosome ten), also referred to as MMAC (mutated in multiple advanced cancers) phosphatase, is a tumor suppressor implicated in a wide variety of human cancers (1). PTEN encodes a 403 amino acid polypeptide originally described as a dual-specificity protein phosphatase (2). The main substrates of PTEN are inositol phospholipids generated by the activation of the phosphoinositide 3-kinase (PI3K) (3). PTEN is a major negative regulator of the PI3K/Akt signaling pathway (1,4,5). PTEN possesses a carboxy-terminal, noncatalytic regulatory domain with three phosphorylation sites (Ser380, Thr382, and Thr383) that regulate PTEN stability and may affect its biological activity (6,7). PTEN regulates p53 protein levels and activity (8) and is involved in G protein-coupled signaling during chemotaxis (9,10).
    Phosphoinositide-dependent protein kinase 1 (PDK1) plays a central role in many signal transduction pathways (11,12), including the activation of Akt and the PKC isoenzymes p70 S6 kinase and RSK (13). Through its effects on these kinases, PDK1 is involved in the regulation of a wide variety of processes, including cell proliferation, differentiation, and apoptosis.
    For Research Use Only. Not For Use In Diagnostic Procedures.
    Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
    XP is a registered trademark of Cell Signaling Technology, Inc.
    U.S. Patent No. 7,429,487, foreign equivalents, and child patents deriving therefrom.
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