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30590
PTEN and PDK1 Antibody Sampler Kit II
Primary Antibodies
Antibody Sampler Kit

PTEN and PDK1 Antibody Sampler Kit II #30590

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PTEN and PDK1 Antibody Sampler Kit II: Image 1

Immunohistochemical analysis of paraffin-embedded human colon using PTEN (D4.3) XP® Rabbit mAb.

PTEN and PDK1 Antibody Sampler Kit II: Image 2

Western blot analysis of extracts from HeLa and NIH/3T3 cells, using Phospho-PTEN (Ser380/Thr382/383) (44A7) Rabbit mAb. Membranes were either left untreated (-) or treated with (+) calf intestinal phosphatase (CIP) post Western transfer to verify phospho-specificity of the antibody.

PTEN and PDK1 Antibody Sampler Kit II: Image 3

Western blot analysis of extracts from various cell lines using PDK1 (D37A7) Rabbit mAb.

PTEN and PDK1 Antibody Sampler Kit II: Image 4

Western blot analysis of extracts from PC3 cells, HCT116 wild-type and HCT116 PDK1 -/- cells using Phospho-PDK1 (Ser241) (C49H2) Rabbit mAb (upper) and Akt (pan) (C67E7) Rabbit mAb #4691 (lower). (HCT116 wild-type and HCT116 PDK1 -/- cells were kindly provided by Dr. Bert Vogelstein, Johns Hopkins University, Baltimore, MD).

PTEN and PDK1 Antibody Sampler Kit II: Image 5

After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO® is added and emits light during enzyme catalyzed decomposition.

PTEN and PDK1 Antibody Sampler Kit II: Image 6

Immunohistochemical analysis using PTEN (D4.3) XP® Rabbit mAb on SignalSlide(TM) PTEN IHC Controls #8106 (paraffin-embedded LNCaP (left) and NIH/3T3 (right) cells).

PTEN and PDK1 Antibody Sampler Kit II: Image 7

Western blot analysis of extracts from HCT 116 wild-type and HCT 116 PDK1-/- cells using PDK1 (D37A7) Rabbit mAb (upper) and Akt (pan) (C67E7) Rabbit mAb #4691 (lower). (HCT 116 wild-type and HCT 116 PDK1-/- cells were kindly provided by Dr. Bert Vogelstein, Johns Hopkins University, Baltimore, MD).

PTEN and PDK1 Antibody Sampler Kit II: Image 8

Western blot analysis of extracts from PC3 cells, untreated or λ phosphatase-treated, using Phospho-PDK1 (Ser241) (C49H2) Rabbit mAb (upper), PDK1 Antibody #3062 (middle) or Akt Antibody #9272 (lower).

PTEN and PDK1 Antibody Sampler Kit II: Image 9

Western blot analysis of extracts from various cell lines using PTEN (D4.3) XP® Rabbit mAb (upper) and Akt (pan) (C67E7) Rabbit mAb #4691 (lower).

To Purchase # 30590T
Product # Size Price
30590T
1 Kit  (4 x 20 µl) $ 327

Product Includes Quantity Applications Reactivity MW(kDa) Isotype
PTEN (D4.3) XP® Rabbit mAb 9188 20 µl
  • WB
  • IP
  • IHC
Dg H Mk M R 54 Rabbit IgG
Phospho-PTEN (Ser380/Thr382/383) (44A7) Rabbit mAb 9549 20 µl
  • WB
H Mk M R 54 Rabbit IgG
PDK1 (D37A7) Rabbit mAb 5662 20 µl
  • WB
Hm H Mk M R 58-68 Rabbit IgG
Phospho-PDK1 (Ser241) (C49H2) Rabbit mAb 3438 20 µl
  • WB
  • IP
H M R 58 to 68 Rabbit IgG
Anti-rabbit IgG, HRP-linked Antibody 7074 100 µl
  • WB
Goat 

Product Description

The PTEN and PDK1 Antibody Sampler Kit II provides an economical means to evaluate two key enzymes that regulate multiple signaling pathways. The kit includes enough antibodies to perform two western blot experiments with each primary antibody.

Specificity / Sensitivity

PTEN (D4.3) XP® Rabbit mAb detects endogenous levels of total PTEN protein. Phospho-PTEN (Ser380/Thr382/383) (44A7) Rabbit mAb detects endogenous levels of PTEN when phosphorylated at Ser380, Thr382 and Thr383. It may also react with PTEN singly phosphorylated at Ser380. PDK1 (D37A7) Rabbit mAb detects endogenous levels of total PDK1 protein. Phospho-PDK1 (Ser241) (C49H2) Rabbit mAb detects PDK1 only when phosphorylated at Ser241.

Source / Purification

PTEN (D4.3) XP® Rabbit mAb is produced by immunizing animals with a synthetic peptide corresponding to residues in the carboxy-terminal sequence of human PTEN. Phospho-PTEN (Ser380/Thr382/383) (44A7) Rabbit mAb is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues around Ser380, Thr382 and Thr383 of human PTEN. PDK1 (D37A7) Rabbit mAb is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human PDK1 protein. Phospho-PDK1 (Ser241) (C49H2) Rabbit mAb is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues around Ser241 of human PDK1.

Background

PTEN (phosphatase and tensin homologue deleted on chromosome ten), also referred to as MMAC (mutated in multiple advanced cancers) phosphatase, is a tumor suppressor implicated in a wide variety of human cancers (1). PTEN encodes a 403 amino acid polypeptide originally described as a dual-specificity protein phosphatase (2). The main substrates of PTEN are inositol phospholipids generated by the activation of the phosphoinositide 3-kinase (PI3K) (3). PTEN is a major negative regulator of the PI3K/Akt signaling pathway (1,4,5). PTEN possesses a carboxy-terminal, noncatalytic regulatory domain with three phosphorylation sites (Ser380, Thr382, and Thr383) that regulate PTEN stability and may affect its biological activity (6,7). PTEN regulates p53 protein levels and activity (8) and is involved in G protein-coupled signaling during chemotaxis (9,10).

Phosphoinositide-dependent protein kinase 1 (PDK1) plays a central role in many signal transduction pathways (11,12), including the activation of Akt and the PKC isoenzymes p70 S6 kinase and RSK (13). Through its effects on these kinases, PDK1 is involved in the regulation of a wide variety of processes, including cell proliferation, differentiation, and apoptosis.

  1. Cantley, L.C. and Neel, B.G. (1999) Proc Natl Acad Sci USA 96, 4240-5.
  2. Myers, M.P. et al. (1997) Proc Natl Acad Sci USA 94, 9052-7.
  3. Myers, M.P. et al. (1998) Proc Natl Acad Sci USA 95, 13513-8.
  4. Wan X and Helman LJ (2003) Oncogene 22, 8205–11
  5. Wu, X. et al. (1998) Proc Natl Acad Sci USA 95, 15587-91.
  6. Vazquez, F. et al. (2000) Mol Cell Biol 20, 5010-8.
  7. Torres, J. and Pulido, R. (2001) J Biol Chem 276, 993-8.
  8. Freeman, D.J. et al. (2003) Cancer Cell 3, 117-30.
  9. Funamoto, S. et al. (2002) Cell 109, 611-23.
  10. Iijima, M. and Devreotes, P. (2002) Cell 109, 599-610.
  11. Belham, C. et al. (1999) Curr Biol 9, R93-6.
  12. Toker, A. and Newton, A.C. (2000) Cell 103, 185-8.
  13. Williams, M.R. et al. (2000) Curr Biol 10, 439-48.

Pathways & Proteins

Explore pathways + proteins related to this product.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST's products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST's Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
XP is a registered trademark of Cell Signaling Technology, Inc.
U.S. Patent No. 7,429,487, foreign equivalents, and child patents deriving therefrom.