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Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

PYCR1 (E7P3I) Rabbit mAb #37635

Filter:
  • WB
  • IP

    Supporting Data

    REACTIVITY H Mk
    SENSITIVITY Endogenous
    MW (kDa) 30, 32
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 
    • Mk-Monkey 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:50

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    PYCR1 (E7P3I) Rabbit mAb recognizes endogenous levels of total PYCR1 protein.

    Species Reactivity:

    Human, Monkey

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala114 of human PYCR1 protein.

    Background

    Mitochondrial pyrroline-5-carboxylate reductase 1 (PYCR1) catalyzes the last step in proline biosynthesis (1). Research studies show that proline is a limiting amino acid to clear cell renal cell carcinoma (ccRCC). PYCR1 expression is induced in kidney cancer cells to compensate for the proline shortage and maintain cancer cell growth. Furthermore, PYCR1 knockdown inhibits ccRCC cell proliferation in the absence of proline, suggesting this enzyme as a potential cancer therapeutic target (2). In addition, breast cancer cells also express high levels of PYCR1 (2,3). Proline is restrictive to invasive ductal breast carcinoma cells, indicating proline vulnerability in breast cancer formation (2).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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