Render Target: STATIC
Render Timestamp: 2025-01-17T12:24:35.470Z
Commit: da7e4f2f0d1aed1f1f8e20e4e2ecab8f33cbd595
XML generation date: 2024-12-02 22:01:09.497
Product last modified at: 2025-01-11T01:00:10.277Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77

Pyk2 (5E2) Mouse mAb #3480

Filter:
  • WB
  • IP

    Supporting Data

    REACTIVITY H M
    SENSITIVITY Endogenous
    MW (kDa) 116
    Source/Isotype Mouse IgG2a
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Simple Western™ 1:50 - 1:250
    Immunoprecipitation 1:50

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    Pyk2 (5E2) Mouse mAb detects endogenous levels of total Pyk2 protein. It does not cross-react with other related proteins.

    Species Reactivity:

    Human, Mouse

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a GST-Pyk2 fusion protein containing residues surrounding the carboxy-terminal of human Pyk2.

    Background

    Protein-tyrosine kinase 2 (Pyk2), also called CAKβ, RAFTK, and CADTK, is a nonreceptor tyrosine kinase structurally related to focal adhesion kinase (FAK) (1-4). Pyk2 is predominantly expressed in cells derived from hematopoietic lineages and in the central nervous system. Pyk2 is one of the signaling mediators for the G protein-coupled receptors and MAP kinase signaling pathway. It plays an important role in cell spreading and migration (5-7).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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