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3962
R-Ras Antibody
Primary Antibodies

R-Ras Antibody #3962

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Supporting Data

REACTIVITY
SENSITIVITY Endogenous
MW (kDa) 24
SOURCE Rabbit

Application Key:

  • W-Western
  • IP-Immunoprecipitation
  • IHC-Immunohistochemistry
  • ChIP-Chromatin Immunoprecipitation
  • IF-Immunofluorescence
  • F-Flow Cytometry
  • E-P-ELISA-Peptide

Species Cross-Reactivity Key:

  • H-Human
  • M-Mouse
  • R-Rat
  • Hm-Hamster
  • Mk-Monkey
  • Mi-Mink
  • C-Chicken
  • Dm-D. melanogaster
  • X-Xenopus
  • Z-Zebrafish
  • B-Bovine
  • Dg-Dog
  • Pg-Pig
  • Sc-S. cerevisiae
  • Ce-C. elegans
  • Hr-Horse
  • All-All Species Expected

Storage:

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Specificity / Sensitivity

R-Ras Antibody detects endogenous levels of total R-Ras protein. The antibody does not cross-react with H-, K-, N- or M-Ras.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy-terminus of human R-Ras. Antibodies are purified by protein A and peptide affinity chromatography.

Background

R-Ras, a member of the small GTPase family, is homologous to H-, K- and N-Ras, but does not activate MAP kinase pathways and is only weakly oncogenic (1). Instead, R-Ras is engaged in integrin activation (2). The effector loop and the carboxy-terminal proline-rich and prenylation sites of R-Ras are critical for integrin activation (3,4). Phosphorylation by EphB2 receptor tyrosine kinase and Src at Tyr66 of R-Ras suppresses integrin activity (5,6).

  1. Self, A. J. et al. (2001) J. Cell Sci. 114, 1357-1366.
  2. Zhang, Z. et al. (1996) Cell 85, 61-69.
  3. Oertli, B. et al. (2000) Oncogene 19, 4961-4969.
  4. Wang, B. et al. (2000) J Biol Chem 275, 5222-7.
  5. Zou, J.X. et al. (1999) Proc Natl Acad Sci U S A 96, 13813-8.
  6. Zou, J.X. et al. (2002) J Biol Chem 277, 1824-7.

Pathways & Proteins

Explore pathways + proteins related to this product.

For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

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