Render Target: STATIC
Render Timestamp: 2024-12-02T10:52:02.388Z
Commit: cd2fae6ca3f811b1ddb1df24ac291ed56d5d501b
XML generation date: 2024-09-30 01:58:08.134
Product last modified at: 2024-10-08T18:30:12.113Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

Rab27B (E4V3O) Rabbit mAb #17572

Filter:
  • WB
  • IP
  • IHC
  • IF

    Supporting Data

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 27
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    • IHC-Immunohistochemistry 
    • IF-Immunofluorescence 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:100
    Immunohistochemistry (Paraffin) 1:200 - 1:800
    Immunofluorescence (Immunocytochemistry) 1:800 - 1:3200

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    Rab27B (E4V3O) Rabbit mAb recognizes endogenous levels of total Rab27B protein. This antibody does not cross-react with Rab27A protein.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly208 of human Rab27B protein.

    Background

    Rab27 proteins are members of the Ras superfamily of small Rab GTPases associated with the process of exocytosis (1-2), and in particular the exosome secretion pathway (3-5). There are two Rab27 isoforms, Rab27A and Rab27B, which are encoded by separate genes and which exhibit distinct subcellular localization and functions. Rab27A colocalizes to CD63+ vesicles, whereas Rab27B is observed in perinuclear vesicles. Targeted knockdown studies suggest that the isoforms control distinct steps of the exosome secretion pathway (6). Rab27A participates in docking and membrane fusion from multivesicular endosomes (MVEs) to exosomes, whereas Rab27B mediates the transfer of membrane vesicles from the trans-Golgi network to MVEs. It has been suggested that Rab27A and Rab27B may have importance in exosome secretion by cancer cells (7).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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