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  3. Rac1/2/3 (L129) Antibody

Rac1/2/3 (L129) Antibody #2467

Inquiry Info. # 2467

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    Product Specifications

    REACTIVITY
    SENSITIVITY Endogenous
    MW (kDa) 21
    SOURCE Rabbit

    Product Information

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

    Specificity / Sensitivity

    Rac1/2/3 (L129) Antibody detects endogenous levels of total Rac1/2/3 proteins.

    Source / Purification

    Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the sequence of human Rac1/2/3. Antibodies are purified using protein A and peptide affinity chromatography.

    Background

    Rac and Cdc42 are members of the Rho-GTPase family. In mammals, Rac exists as three isoforms, Rac1, Rac2 and Rac3, which are highly similar in sequence. Rac1 and Cdc42, the most widely studied of this group, are ubiquitously expressed. Rac2 is expressed in cells of hematopoietic origin, and Rac3, while highly expressed in brain, is also found in many other tissues. Rac and Cdc42 play key signaling roles in cytoskeletal reorganization, membrane trafficking, transcriptional regulation, cell growth and development (1). GTP binding stimulates the activity of Rac/Cdc42, and the hydrolysis of GTP to GDP through the protein's intrinsic GTPase activity, rendering it inactive. GTP hydrolysis is aided by GTPase activating proteins (GAPs), while exchange of GDP for GTP is facilitated by guanine nucleotide exchange factors (GEFs). Another level of regulation is achieved through the binding of RhoGDI, a guanine nucleotide dissociation inhibitor, which retains Rho family GTPases, including Rac and Cdc42, in their inactive GDP-bound state (2,3).

    Alternate Names

    Cell migration-inducing gene 5 protein; EN-7; GX; HSPC022; IMD73A; IMD73B; IMD73C; MGC111543; MIG5; migration-inducing gene 5; MRD48; p21-Rac1; p21-Rac2; p21-Rac3; Rac family small GTPase 1; Rac family small GTPase 2; Rac family small GTPase 3; Rac-1; RAC1; RAC2; RAC3; Ras-like protein TC25; Ras-related C3 botulinum toxin substrate 1; ras-related C3 botulinum toxin substrate 1 (rho family, small GTP binding protein Rac1); Ras-related C3 botulinum toxin substrate 2; ras-related C3 botulinum toxin substrate 2 (rho family, small GTP binding protein Rac2); Ras-related C3 botulinum toxin substrate 3; ras-related C3 botulinum toxin substrate 3 (rho family, small GTP binding protein Rac3); Ras-related C3 botulinum toxin substrate 3 (rho family, small GTP-binding protein Rac2); rho family, small GTP binding protein Rac1; rho family, small GTP binding protein Rac2; rho family, small GTP binding protein Rac3; Small G protein; TC-25; TC25

    Database Links

    UniProt ID: P15153 , P63000 , P60763


    Entrez-Gene Id: 5880 , 5879 , 5881


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