Render Target: STATIC
Render Timestamp: 2024-12-09T11:21:58.506Z
Commit: 224419269841c11382c4555dbee545259bf6c379
XML generation date: 2024-11-18 16:03:13.277
Product last modified at: 2024-11-19T09:00:36.178Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

Rad51 (F1G6C) Rabbit mAb #65653

Filter:
  • WB
  • IF

    Supporting Data

    REACTIVITY H R
    SENSITIVITY Endogenous
    MW (kDa) 37
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IF-Immunofluorescence 
    Species Cross-Reactivity Key:
    • H-Human 
    • R-Rat 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunofluorescence (Immunocytochemistry) 1:200 - 1:800

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    Rad51 (F1G6C) Rabbit mAb recognizes endogenous levels of total Rad51 protein.

    Species Reactivity:

    Human, Rat

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human Rad51 protein.

    Background

    DNA double-strand breaks (DSBs) are potentially hazardous lesions that can be induced by ionizing radiation (IR), radiomimetic chemicals, or DNA replication inhibitors. Cells sense and repair DSBs via two distinct but partly overlapping signaling pathways: nonhomologous end joining (NHEJ) and homologous recombination (HR). Research studies have shown that defects in both pathways are associated with human diseases, including cancer (reviewed in 1).

    DSBs that arise during S or G2 phase are repaired via homologous recombination (HR), using the replicated sister chromatid as a repair template. Rad51 recombinase, a eukaryotic homolog of E. coli RecA, polymerizes and forms a filament along single-stranded DNA, mediating HR with the help of auxiliary proteins, including Rad54 and BRCA2 (reviewed in 2,3). BRCA2 binds Rad51 and targets it to single-stranded DNA, allowing it to displace replication protein A (RPA) (4). Five Rad51 paralogs exist in vertebrates (XRCC2, XRCC3, Rad51B, Rad51C, and Rad51D), and they all appear to be required for efficient HR (5).

    Researchers have found that mutations in the Rad51 gene may be related to breast cancer risk (6). Some studies have implicated Rad51 as a potential marker for pancreatic cancer (7).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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