Render Target: STATIC
Render Timestamp: 2024-12-12T11:45:31.132Z
Commit: 611277b6de3cd1bb065350b6ef8d63df412b7185
XML generation date: 2024-09-30 01:54:36.517
Product last modified at: 2024-09-30T08:01:45.761Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77

Rap1B (36E1) Rabbit mAb #2326

Filter:
  • WB

    Supporting Data

    REACTIVITY H M R Mk B Pg
    SENSITIVITY Endogenous
    MW (kDa) 21
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 
    • Mk-Monkey 
    • B-Bovine 
    • Pg-Pig 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    Rap1B (36E1) Rabbit mAb detects endogenous levels of total Rap1B protein. It does not cross-react with Rap1A.

    Species Reactivity:

    Human, Mouse, Rat, Monkey, Bovine, Pig

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with synthetic peptides corresponding to the carboxy terminal half of human Rap1B.

    Background

    Rap1 and Rap2 belong to the Ras subfamily of small GTPases and are activated by a wide variety of stimuli through integrins, receptor tyrosine kinases (RTKs), G-protein coupled receptors (GPCR), death domain associated receptors (DD-R) and ion channels (1,2). Like other small GTPases, Rap activity is stimulated by guanine nucleotide exchange factors (GEF) and inactivated by GTPase activating proteins (GAP). A wide variety of Rap GEFs have been identified: C3G connects Rap1 with RTKs through adaptor proteins such as Crk, Epacs (or cAMP-GEFs) transmit signals from cAMP, and CD-GEFs (or CalDAG-GEFs) convey signals from either or both Ca2+ and DAG (1). Rap1 primarily regulates multiple integrin-dependent processes such as morphogenesis, cell-cell adhesion, hematopoiesis, leukocyte migration and tumor invasion (1,2). Rap1 may also regulate proliferation, differentiation and survival through downstream effectors including B-Raf, PI3K, RalGEF and phospholipases (PLCs) (1-4). Rap1 and Rap2 are not fuctionally redundant as they perform overlapping but distinct functions (5). Recent research indicates that Rap2 regulates Dsh subcellular localization and is required for Wnt signaling in early development (6).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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