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32307
PhosphoPlus® Raptor (Ser792) Antibody Duet
Primary Antibodies
Antibody Duet

PhosphoPlus® Raptor (Ser792) Antibody Duet #32307

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PhosphoPlus® Raptor (Ser792) Antibody Duet: Image 1

Immunoprecipitation of raptor protein from MCF7 cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is Raptor (E6O3A) Rabbit mAb. Western blot analysis was performed using Raptor (E6O3A) Rabbit mAb. Mouse Anti-rabbit IgG (Conformation Specific) (L27A9) mAb (HRP Conjugate) #5127 was used as the secondary antibody.

PhosphoPlus® Raptor (Ser792) Antibody Duet: Image 2

Immunoprecipitation of phospho-raptor protein from PANC-1 extracts treated with carbonyl cyanide m-chlorophenylhydrazone (CCCP) (100 μM, 2 hr). Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is Phospho-Raptor (Ser792) (E4V6C) Rabbit mAb. Western blot analysis was performed using Phospho-Raptor (Ser792) (E4V6C) Rabbit mAb. Mouse Anti-rabbit IgG (Conformation Specific) (L27A9) mAb (HRP Conjugate) #5127 was used as the secondary antibody.

PhosphoPlus® Raptor (Ser792) Antibody Duet: Image 3

Western blot analysis of extracts from various cell lines using Raptor (E6O3A) Rabbit mAb.

PhosphoPlus® Raptor (Ser792) Antibody Duet: Image 4

Western blot analysis of extracts from PANC-1 cells, vehicle-treated (-) or treated (+) with combinations of the following treatments as indicated: carbonyl cyanide m-chlorophenylhydrazone (CCCP) (100 μM, 2 hr) and λ phosphatase/calf intestinal alkaline phosphatase (CIP), using Phospho-Raptor (Ser792) (E4V6C) Rabbit mAb (upper) or Raptor (24C12) Rabbit mAb #2280 (lower).

PhosphoPlus® Raptor (Ser792) Antibody Duet: Image 5

Western blot analysis of extracts from C2C12 cells, vehicle-treated (-) or treated with carbonyl cyanide m-chlorophenylhydrazone (CCCP) (10 μg/mL, 30 min; +), using Phospho-Raptor (Ser792) (E4V6C) Rabbit mAb (upper) or Raptor (24C12) Rabbit mAb #2280 (lower).

To Purchase # 32307S
Product # Size Price
32307S
1 Kit $ 514

Product Includes Quantity Reactivity MW(kDa) Isotype
Raptor (E6O3A) Rabbit mAb 48648 100 µl H M R Mk 150 Rabbit IgG
Phospho-Raptor (Ser792) (E4V6C) Rabbit mAb 89146 100 µl H M R 150 Rabbit IgG

Product Description

PhosphoPlus® Duets from Cell Signaling Technology (CST) provide a means to assess protein activation status. Each Duet contains an activation-state and total protein antibody to your target of interest. These antibodies have been selected from CST's product offering based upon superior performance in specified applications.

Background

The regulatory associated protein of mTOR (Raptor) was identified as an mTOR binding partner that mediates mTOR signaling to downstream targets (1,2). Raptor binds to mTOR substrates, including 4E-BP1 and p70 S6 kinase, through their TOR signaling (TOS) motifs and is required for mTOR-mediated phosphorylation of these substrates (3,4). Binding of the FKBP12-rapamycin complex to mTOR inhibits the mTOR-raptor interaction, suggesting a mechanism for rapamycin's specific inhibition of mTOR signaling (5). This mTOR-raptor interaction and its regulation by nutrients and/or rapamycin is dependent on a protein called GβL (6). GβL is also part of the rapamycin-insensitive complex between mTOR and rictor (rapamycin-insensitive companion of mTOR), and may mediate rictor-mTOR signaling to downstream targets including PKCα (7). Furthermore, the rictor-mTOR complex has been identified as the previously elusive PDK2 responsible for the phosphorylation of Akt/PKB on Ser473, facilitating phosphorylation of Akt/PKB on Thr308 by PDK1 and required for the full activation of Akt/PKB (8).

Recently raptor has been identified as a direct substrate of the AMP-activated protein kinase (AMPK) (9). AMPK phosphorylates raptor on Ser722/Ser792 (9). This phosphorylation is essential for inhibition of the raptor-containing mTOR complex 1 (mTORC1) and induces cell cycle arrest when cells are stressed for energy (9). These findings suggest that raptor is a critical switch that correlates cell cycle progression with energy status.

  1. Hara, K. et al. (2002) Cell 110, 177-89.
  2. Kim, D.H. et al. (2002) Cell 110, 163-75.
  3. Beugnet, A. et al. (2003) J Biol Chem 278, 40717-22.
  4. Nojima, H. et al. (2003) J Biol Chem 278, 15461-4.
  5. Oshiro, N. et al. (2004) Genes Cells 9, 359-66.
  6. Kim, D. H. et al. (2003) Mol. Cell 11, 895-904.
  7. Sarbassov, D. et al. (2004) Curr. Biol. 14, 1296-302.
  8. Sarbassov, D.D. et al. (2005) Science 307, 1098-101.
  9. Gwinn, D.M. et al. (2008) Mol Cell 30, 214-26.

Pathways & Proteins

Explore pathways + proteins related to this product.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST's products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST's Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
PhosphoPlus is a trademark of Cell Signaling Technology, Inc.