Render Target: STATIC
Render Timestamp: 2024-12-03T11:17:46.631Z
Commit: cd2fae6ca3f811b1ddb1df24ac291ed56d5d501b
XML generation date: 2024-10-31 20:01:07.461
Product last modified at: 2024-11-01T07:01:09.072Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

RBM10 (E5V5K) Rabbit mAb #47729

Filter:
  • WB
  • IP
  • eCLIP

    Supporting Data

    REACTIVITY H Mk
    SENSITIVITY Endogenous
    MW (kDa) 110, 135
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    • eCLIP-eCLIP 
    Species Cross-Reactivity Key:
    • H-Human 
    • Mk-Monkey 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:50
    eCLIP 1:200
    For more information about the RBP-eCLIP service please visit Eclipsebio.

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    RBM10 (E5V5K) Rabbit mAb recognizes endogenous levels of total RBM10 protein.

    Species Reactivity:

    Human, Monkey

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding His919 of human RBM10 protein.

    Background

    RBM10 is an RNA binding protein and a paralog to tumor suppressor RBM5 (1,2). Alternative splicing creates two highly expressed variants that differ by one exon. RBM10 controls alternative splicing and exon skipping of mRNAs, including Fas and Bcl-x (3,4). RBM10 has been shown to affect apoptosis via up regulation of TNF-α mRNA (5). In lung cancer, RBM10 mutations disrupt the splicing of NUMB, a regulator of Notch signaling (6,7). Fusion of the RBM10 gene with TFE3 has been found in renal cell carcinoma (8-9). Mutations of RBM10 causes TARP syndrome, an x-linked, lethal disorder characterized by various developmental defects (10-12).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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