RBM15 (E8Y8A) Rabbit mAb #60386
- WB
- IP
- eCLIP
Supporting Data
| REACTIVITY | H M R Mk |
| SENSITIVITY | Endogenous |
| MW (kDa) | 110 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
- eCLIP-eCLIP
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
- Mk-Monkey
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
| eCLIP | 1:200 |
Storage
Protocol
Specificity / Sensitivity
RBM15 (E8Y8A) Rabbit mAb recognizes endogenous levels of total RBM15 protein.
Species Reactivity:
Human, Mouse, Rat, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala557 of human RBM15 protein.
Background
RBM15 is an RNA-binding protein that is part of the WTAP-METTL3 m6A methyltransferase complex. RBM15 and related RBM15B interact with WTAP to recruit the complex to target mRNAs, and are critical to XIST-mediated gene silencing (1). RBM15 can recruit splicing factors such as SF3B1 to mRNA to promote alternative splicing. Expression levels of RBM15 can be regulated by PRMT1, which can methylate R578, resulting in RBM15 ubiquitinylation and degradation (2). This process is critical in acute megakaryoblastic leukemia, a cancer type where RBM15 is fused to the MKL-1 gene and PRMT1 is overexpressed (3). RBM15 normally plays roles in hematopoietic development and myeloid differentiation, where it can regulate the levels of c-Myc (4-6).
- Patil, D.P. et al. (2016) Nature 537, 369-73.
- Zhang, L. et al. (2015) Elife 4, e07938. doi: 10.7554/eLife.07938.
- Ma, Z. et al. (2001) Nat Genet 28, 220-1.
- Ma, X. et al. (2007) Mol Cell Biol 27, 3056-64.
- Raffel, G.D. et al. (2007) Proc Natl Acad Sci U S A 104, 6001-6.
- Niu, C. et al. (2009) Blood 114, 2087-96.
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