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8589
Retinoic Acid and Retinoid X Receptors Antibody Sampler Kit
Primary Antibodies

Retinoic Acid and Retinoid X Receptors Antibody Sampler Kit #8589

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Western Blotting Image 1

Western blot analysis of extracts from various cell lines using RXRα (D6H10) Rabbit mAb.

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Western Blotting Image 2

Western blot analysis of extracts from NIH/3T3 and C6 cells, using RARα Antibody.

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Western Blotting Image 3

Western blot analysis of extracts from various cell lines using RXRβ Antibody.

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Western Blotting Image 4

Western blot analysis of extracts from various cell lines using RARγ1 (D3A4) XP® Rabbit mAb.

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Western Blotting Image 5

Western blot analysis of extracts from 293T cells, either mock transfected or transfected with human RXRα, RXRβ, or RXRγ DYKDDDK-tagged constructs, using RXRγ Antibody (upper) and DYKDDDK Tag Antibody (Binds to same epitope as Sigma's Anti-FLAG® M2 Antibody) #2368 (lower).

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Western Blotting Image 6

After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO® is added and emits light during enzyme catalyzed decomposition.

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Isoform Specificity Image 7

Western blot analysis of extracts from 293T cells, either mock transfected (-) or transfected with Myc/DDK-tagged cDNA expression constructs encoding full-length human RXRα (hRXRα; +), RXRβ (hRXRβ; +), or RXRγ (hRXRγ; +), using RXRα (D6H10) Rabbit mAb (upper) and DYKDDDDK Tag Antibody (Binds to same epitope as Sigma's Anti-FLAG® M2 Antibody) #2368 (lower).

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Isoform Specificity Image 8

Western blot analysis of extracts from 293T cells, either mock-transfected or transfected with a Myc/DDK-tagged cDNA expression construct encoding full-length human RXRα, RXRβ, and RXRγ, using RXRβ Antibody (upper) or DYKDDDDK Tag Antibody (Binds to same epitope as Sigma's Anti-FLAG® M2 Antibody) #2368 (lower).

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Western Blotting Image 9

Western blot analysis of extracts from 293T cells, either mock transfected (-) or transfected with a Myc/DDK-tagged cDNA expression construct encoding full-length human RARγ1 (hRARγ1-Myc/DDK, +), using RARγ1 (D3A4) XP® Rabbit mAb.

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Western Blotting Image 10

Western blot analysis of extracts from various tissues using RXRγ Antibody.

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IHC-P (paraffin) Image 11

Immunohistochemical analysis of paraffin-embedded cell pellets, HaCaT (positive, left) and Hep3B (negative, right), using RARγ1 (D3A4) XP® Rabbit mAb.

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IHC-P (paraffin) Image 12

Immunohistochemical analysis of paraffin-embedded human lung carcinoma using RARγ1 (D3A4) XP® Rabbit mAb.

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IHC-P (paraffin) Image 13

Immunohistochemical analysis of paraffin-embedded human skin using RARγ1 (D3A4) XP® Rabbit mAb.

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Flow Cytometry Image 14

Flow cytometric analysis of T-47D cells using RARγ1 (D3A4) XP® Rabbit mAb (blue) compared to concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (red). Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.

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IF-IC Image 15

Confocal immunofluorescent analysis of HaCaT cells (positive, left) and Hep3B cells (negative, right) using RARγ1 (D3A4) XP® Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red).

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Product Includes Quantity Applications Reactivity MW(kDa) Isotype
RXRα (D6H10) Rabbit mAb 3085 20 µl
  • WB
  • IP
H M R 53 Rabbit IgG
RARα Antibody 2554 20 µl
  • WB
M R 55 Rabbit 
RXRβ Antibody 8715 20 µl
  • WB
  • IP
H M 70-72 Rabbit 
RARγ1 (D3A4) XP® Rabbit mAb 8965 20 µl
  • WB
  • IP
  • IHC
  • IF
  • F
H M 58 Rabbit IgG
RXRγ Antibody 5629 20 µl
  • WB
  • IP
H M 55 Rabbit 
Anti-rabbit IgG, HRP-linked Antibody 7074 100 µl
  • WB
Goat 

The Retinoic Acid and Retinoid X Receptors Antibody Sampler Kit provides an economical means to investigate the expression of various subtypes of retinoic acid and retinoid X receptors. The kit contains enough primary antibody to perform two western blot experiments per primary.

Each antibody in the Retinoic Acid and Retinoid X Receptors Antibody Sampler Kit recognizes endogenous levels of total respective protein. The antibodies do not cross react with other subtypes of retinoic acid or retinoic X receptors.

Monoclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human RARγ1 protein or human RXRα protein. Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the sequence of human RARα protein, residues near the amino terminus of human RXRβ protein, or residues near the amino terminus of human RXRγ protein. Polyclonal antibodies are purified by protein A and peptide affinity chromatography.

Nuclear retinoic acid (RA) receptors (RARs) consist of three subtypes encoded by separate genes: α (NR1B1), β (NR1B2), and γ (NR1B3). For each subtype, there are at least two isoforms, which are generated by differential promoter usage and alternative splicing and differ only in their N-terminal regions. Retinoids, which are metabolites of vitamin A, serve as ligands for RARs (1). RARs function as ligand-dependent transcriptional regulators and are found to be heterodimerized with retinoid X receptors (RXRs). These transcriptionally active dimers regulate the expression of genes involved in cellular differentiation, proliferation, and apoptosis (2,3). Consequently, RARs play critical roles in a variety of biological processes, including development, reproduction, immunity, and organogenesis (4-6). RAR mutations, fusion proteins, altered expression levels, or aberrant post-translational modifications result in multiple diseases due to altered RAR function and disruption of homeostasis.

In contrast to the ubiquitously expressed RARα subtype, RARγ displays a complex tissue-specific expression pattern (7). The hematopoietic system expresses significant levels of RARγ, and a recent study identified a role for RARγ in hematopoietic stem cell maintenance (8). RARγ is the predominant subtype in human and mouse epidermis, representing 90% of the RARs in this tissue (9-11). Given the high level of RARγ expression in the skin, it has been suggested that this nuclear receptor participates in a transcriptional program that governs maintenance and differentiation of normal epidermis and skin appendages. The transcriptional activity of RARγ is under stringent control, in part, through retinoic acid-induced phosphorylation and proteasomal degradation (12).

The human retinoid X receptors (RXRs) are encoded by three distinct genes (RXRα, RXRβ, and RXRγ) and bind selectively and with high affinity to the vitamin A derivative, 9-cis-retinoic acid. RXRs are type-II nuclear hormone receptors that are largely localized to the nuclear compartment independent of ligand binding. Nuclear RXRs form heterodimers with nuclear hormone receptor subfamily 1 proteins, including thyroid hormone receptor, retinoic acid receptors, vitamin D receptor, peroxisome proliferator-activated receptors, liver X receptors, and farnesoid X receptor (13). Since RXRs heterodimerize with multiple nuclear hormone receptors, they play a central role in transcriptional control of numerous hormonal signaling pathways by binding to cis-acting response elements in the promoter/enhancer region of target genes (14).

  1. Mangelsdorf, D.J. et al. (1992) Genes Dev 6, 329-44.
  2. Mark, M. et al. (2006) Annu Rev Pharmacol Toxicol 46, 451-80.
  3. Niederreither, K. and Dollé, P. (2008) Nat Rev Genet 9, 541-53.
  4. Mark, M. et al. (2009) Nucl Recept Signal 7, e002.
  5. Dollé, P. (2009) Nucl Recept Signal 7, e006.
  6. Purton, L.E. et al. (2006) J Exp Med 203, 1283-93.
  7. Fisher, G.J. et al. (1994) J Biol Chem 269, 20629-35.
  8. Zelent, A. et al. (1989) Nature 339, 714-7.
  9. Elder, J.T. et al. (1991) J Invest Dermatol 96, 425-33.
  10. Giannì, M. et al. (2002) EMBO J 21, 3760-9.
  11. Rochette-Egly, C. and Germain, P. (2009) Nucl Recept Signal 7, e005.
  12. Delacroix, L. et al. (2010) Mol Cell Biol 30, 231-44.
  13. Eifert, C. et al. (2006) Mol Reprod Dev 73, 796-824.
  14. Gronemeyer, H. et al. (2004) Nat Rev Drug Discov 3, 950-64.
Entrez-Gene Id
5914 , 5916 , 6256 , 6257 , 6258
Swiss-Prot Acc.
P10276 , P13631 , P19793 , P28702 , P48443
For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
XP is a registered trademark of Cell Signaling Technology, Inc.
Anti-FLAG is a registered trademark of Sigma-Aldrich Biotechnology.

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