Render Target: STATIC
Render Timestamp: 2025-02-18T11:12:47.117Z
Commit: 7500bcdc731e9059bbdfbdbe9e72caa896e426e8
XML generation date: 2024-09-30 01:54:25.317
Product last modified at: 2025-01-05T17:15:07.662Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

RGS4 (D4V1P) Rabbit mAb #15129

Filter:
  • WB
  • IP

    Supporting Data

    REACTIVITY H M R
    SENSITIVITY Endogenous
    MW (kDa) 25
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:50

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    RGS4 (D4V1P) Rabbit mAb recognizes endogenous levels of total RGS4 protein.

    Species Reactivity:

    Human, Mouse, Rat

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human RGS4 protein.

    Background

    Regulators of G protein signaling (RGS) are a family of about 40 proteins that determine the signaling amplitude and duration of G protein-coupled receptor signaling via modulation of the GTPase activity of G proteins (1-3). Each RGS has a distinct structure, expression pattern, and regulation, resulting in preferential interactions with receptors, G proteins, and other signaling proteins, as well as a unique function (4).
    Regulator of G-protein signaling 4 (RGS4) is a small RGS (23 kDa) that belongs to the R4 subfamily of heterotrimeric G-protein regulators. RGS4 contains a short amino-terminus that may be responsible for its functional specificity towards G-protein-coupled receptors, and a conserved RGS domain responsible for GAP activity (1). The RGS4 protein shares activity at both Gαq and Gαi/o proteins (5,6). Degradation of RGS4 is regulated by opioid receptor-mediated ubiquitination following stimulation by opioid receptor agonists, such as the DAMGO synthetic opioid peptide (7). RGS4 modulates the action of morphine and other opiates in vivo (8).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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