|2217||S6 Ribosomal Protein (5G10) Rabbit mAb||
||H M R Mk|
|2317||S6 Ribosomal Protein (54D2) Mouse mAb||
||H M R Mk Dm|
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
S6 Ribosomal Protein Antibody detects endogenous levels of total S6 ribosomal protein independent of phosphorylation.
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues 185-205 of human S6 ribosomal protein. Antibodies are purified by protein A and peptide affinity chromatography.
One way that growth factors and mitogens effectively promote sustained cell growth and proliferation is by upregulating mRNA translation (1,2). Growth factors and mitogens induce the activation of p70 S6 kinase and the subsequent phosphorylation of the S6 ribosomal protein. Phosphorylation of S6 ribosomal protein correlates with an increase in translation of mRNA transcripts that contain an oligopyrimidine tract in their 5' untranslated regions (2). These particular mRNA transcripts (5'TOP) encode proteins involved in cell cycle progression, as well as ribosomal proteins and elongation factors necessary for translation (2,3). Important S6 ribosomal protein phosphorylation sites include several residues (Ser235, Ser236, Ser240, and Ser244) located within a small, carboxy-terminal region of the S6 protein (4,5).
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