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57295
PhosphoPlus® Rictor (Thr1135) Antibody Duet

PhosphoPlus® Rictor (Thr1135) Antibody Duet #57295

Western Blotting Image 1

Western blot analysis of extracts from serum-starved HeLa cells, untreated or treated with IGF-1 (50 ng/ml, 30 minutes) and λ phosphatase, using Phospho-Rictor (Thr1135) (D30A3) Rabbit mAb (upper) or Rictor (53A2) Rabbit mAb #2114 (lower).

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Western Blotting Image 2

Western blot analysis of extracts from various cell lines, using Rictor (53A2) Rabbit mAb.

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Product Includes Quantity Applications Reactivity MW(kDa) Isotype
Phospho-Rictor (Thr1135) (D30A3) Rabbit mAb 3806 100 µl
  • WB
H M Mk 200 Rabbit IgG
Rictor (53A2) Rabbit mAb 2114 100 µl
  • WB
H M R Mk 200 Rabbit IgG

PhosphoPlus® Duets from Cell Signaling Technology (CST) provide a means to assess protein activation status. Each Duet contains an activation-state and total protein antibody to your target of interest. These antibodies have been selected from CST's product offering based upon superior performance in specified applications.

Cell growth is a fundamental biological process whereby cells accumulate mass and increase in size. The mammalian TOR (mTOR) pathway regulates growth by coordinating energy and nutrient signals with growth factor-derived signals (1). mTOR is a large protein kinase with two different complexes. One complex contains mTOR, GβL and raptor, which is a target of rapamycin. The other complex, insensitive to rapamycin, includes mTOR, GβL, Sin1, and rictor (1). The mTOR-rictor complex phosphorylates Ser473 of Akt/PKB in vitro (2). This phosphorylation is essential for full Akt/PKB activation. Furthermore, an siRNA knockdown of rictor inhibits Ser473 phosphorylation in 3T3-L1 adipocytes (3). This complex has also been shown to phosphorylate the rapamycin-resistant mutants of S6K1, another effector of mTOR (4).

Phosphorylation of Thr1135 on rictor was identified at Cell Signaling Technology (CST) using PhosphoScan®, CST's LC-MS/MS platform for phosphorylation site discovery (5). Additional research indicates that rictor is phosphorylated at Thr1135 by p70 S6K, which negatively regulates mTORC2 protein complex as part of a negative feedback mechanism controlling Akt activity (6-8).

  1. Sarbassov, D.D. et al. (2004) Curr. Biol. 14, 1296-1302.
  2. Sarbassov, D.D. et al. (2005) Science 307, 1098-1101.
  3. Hresko, R.C. and Mueckler, M. (2005) J. Biol. Chem. 280, 40406-40416.
  4. Ali, S.M. and Sabatini, D.M. (2005) J. Biol. Chem. 280, 19445-19448.
  5. Dibble, C.C. et al. (2009) Mol Cell Biol 29, 5657-70.
  6. Julien, L.A. et al. (2010) Mol Cell Biol 30, 908-21.
  7. Rush, J. et al. (2005) Nat Biotechnol 23, 94-101.
  8. Treins, C. et al. (2010) Oncogene 29, 1003-16.
Entrez-Gene Id
253260
Swiss-Prot Acc.
Q6R327
For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
CST is a trademark of Cell Signaling Technology, Inc.
PhosphoPlus is a trademark of Cell Signaling Technology, Inc.
U.S. Patent No. 7,429,487, foreign equivalents, and child patents deriving therefrom.

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