Rpb1 NTD (D8L4Y) Rabbit mAb #14958
- WB
- ChIP
Supporting Data
REACTIVITY | H M R Mk |
SENSITIVITY | Endogenous |
MW (kDa) | 250 |
Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- ChIP-Chromatin Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
- Mk-Monkey
Product Information
Product Usage Information
For optimal ChIP and ChIP-seq results, use 10 μl of antibody and 10 μg of chromatin (approximately 4 x 106 cells) per IP. This antibody has been validated using SimpleChIP® Enzymatic Chromatin IP Kits.
Application | Dilution |
---|---|
Western Blotting | 1:1000 |
Chromatin IP | 1:50 |
Chromatin IP-seq | 1:50 |
Storage
Protocol
Specificity / Sensitivity
Species Reactivity:
The antigen sequence used to produce this antibody shares 100% sequence homology with the species listed here, but reactivity has not been tested or confirmed to work by CST. Use of this product with these species is not covered under our Product Performance Guarantee.
Species predicted to react based on 100% sequence homology:
Source / Purification
Background
In addition to Ser2/Ser5 phosphorylation, Ser7 of the CTD heptapeptide repeat is also phosphorylated during the active transcription cycle. Phosphorylation at Ser7 is required for efficient transcription of small nuclear (sn) RNA genes (9,10). snRNA genes, which are neither spliced nor poly-adenylated, are structurally different from protein-coding genes. Instead of a poly(A) signal found in protein-coding RNAs, snRNAs contain a conserved 3'-box RNA processing element, which is recognized by the Integrator snRNA 3' end processing complex (11,12). Phosphorylation at Ser7 by CDK7 during the early stages of transcription facilitates recruitment of RPAP2, which dephosphorylates Ser5, creating a dual Ser2/Ser7 phosphorylation mark that facilitates recruitment of the Integrator complex and efficient processing of nascent snRNA transcripts (13-15).
- Brookes, E. and Pombo, A. (2009) EMBO Rep 10, 1213-9.
- Komarnitsky, P. et al. (2000) Genes Dev 14, 2452-60.
- Ho, C.K. and Shuman, S. (1999) Mol Cell 3, 405-11.
- Ng, H.H. et al. (2003) Mol Cell 11, 709-19.
- Cheng, B. and Price, D.H. (2007) J Biol Chem 282, 21901-12.
- Marshall, N.F. et al. (1996) J Biol Chem 271, 27176-83.
- Krogan, N.J. et al. (2003) Mol Cell Biol 23, 4207-18.
- Proudfoot, N.J. et al. (2002) Cell 108, 501-12.
- Chapman, R.D. et al. (2007) Science 318, 1780-2.
- Egloff, S. et al. (2007) Science 318, 1777-9.
- Egloff, S. et al. (2008) Biochem Soc Trans 36, 590-4.
- Baillat, D. et al. (2005) Cell 123, 265-76.
- Akhtar, M.S. et al. (2009) Mol Cell 34, 387-93.
- Egloff, S. et al. (2010) J Biol Chem 285, 20564-9.
- Egloff, S. et al. (2012) Mol Cell 45, 111-22.
Limited Uses
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