RPL15 (E3X8M) Rabbit mAb #29753

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Supporting Data

REACTIVITY	H M R
SENSITIVITY	Endogenous
MW (kDa)	24
Source/Isotype	Rabbit IgG

Application Key:

WB-Western Blotting

Species Cross-Reactivity Key:

H-Human
M-Mouse
R-Rat

Product Information

Product Usage Information

Application	Dilution
Western Blotting	1:1000

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

RPL15 (E3X8M) Rabbit mAb recognizes endogenous levels of total RPL15 protein. This antibody detects a nonspecific signal around 34 kDa in lysates of some cell lines.

Species Reactivity:

Human, Mouse, Rat

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human RPL15 protein.

Background

Ribosomal protein L15 (RPL15) is a structural component of the large 60S subunit of the ribosome. Studies show that overexpressed RPL15 protein promotes metastatic growth of breast cancer patient-derived circulating tumor cells. Overexpression of RPL15 was shown to increase translation of other ribosomal proteins of large and small subunits and cell cycle regulators (1). Furthermore, the DNA topoisomerase I inhibitor topotecan binds to RPL15 and suppresses preribosomal subunit formation, which causes ribosomal stress and enhances DNA topoisomerase I-independent antitumor immune activation mediated by damage-associated molecular patterns (2). In addition, RPL15 expression is upregulated in colon cancer cells. RPL15 deficiency leads to ribosomal stress and, therefore, apoptosis of colon cancer cells (3).

Database Links

UniProt ID: P61313

Entrez-Gene Id: 6138

Limited Uses

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For Research Use Only. Not For Use In Diagnostic Procedures.

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