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S100A4 (D9F9D) Rabbit mAb (BSA and Azide Free)
Primary Antibodies
Monoclonal Antibody

S100A4 (D9F9D) Rabbit mAb (BSA and Azide Free) #48842

Citations (0)
  1. WB
  2. IHC
Western blot analysis of extracts from A172 and HeLa cells using S100A4 (D9F9D) Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemical analysis of paraffin-embedded human colon using S100A4 (D9F9D) Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right). Data were generated using the standard formulation of this product.
Immunohistochemical analysis of paraffin-embedded human prostate carcinoma using S100A4 (D9F9D) Rabbit mAb. Data were generated using the standard formulation of this product.
To Purchase # 48842
Cat. # Size Qty. Price
100 µg

Supporting Data

MW (kDa) 12
Source/Isotype Rabbit IgG

Application Key:

  • WB-Western Blot
  • IP-Immunoprecipitation
  • IHC-Immunohistochemistry
  • ChIP-Chromatin Immunoprecipitation
  • C&T-CUT&Tag
  • DB-Dot Blot
  • IF-Immunofluorescence
  • F-Flow Cytometry

Species Cross-Reactivity Key:

  • H-Human
  • M-Mouse
  • R-Rat
  • Hm-Hamster
  • Mk-Monkey
  • Vir-Virus
  • Mi-Mink
  • C-Chicken
  • Dm-D. melanogaster
  • X-Xenopus
  • Z-Zebrafish
  • B-Bovine
  • Dg-Dog
  • Pg-Pig
  • Sc-S. cerevisiae
  • Ce-C. elegans
  • Hr-Horse
  • GP-Guinea Pig
  • Rab-Rabbit
  • All-All Species Expected

Product Usage Information

This product is the carrier free version of product #13018. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol.

This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us. Optimal dilutions/concentrations should be determined by the end user.


Supplied in 1X PBS, BSA and Azide Free.

For standard formulation of this product see product #13018


Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance.

Specificity / Sensitivity

S100A4 (D9F9D) Rabbit mAb (BSA and Azide Free) recognizes endogenous levels of total S100A4 protein.

Species Reactivity:

Human, Mouse

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala54 of human S100A4 protein.


Despite their relatively small size (8-12 kDa) and uncomplicated architecture, S100 proteins regulate a variety of cellular processes, such as cell growth and motility, cell cycle progression, transcription, and differentiation. To date, 25 members have been identified, including S100A1-S100A18, trichohyalin, filaggrin, repetin, S100P, and S100Z, making it the largest group in the EF-hand, calcium-binding protein family. Interestingly, 14 S100 genes are clustered on human chromosome 1q21, a region of genomic instability. Research studies have demonstrated that significant correlation exists between aberrant S100 protein expression and cancer progression. S100 proteins primarily mediate immune responses in various tissue types but are also involved in neuronal development (1-4).

Each S100 monomer bears two EF-hand motifs and can bind up to two molecules of calcium (or other divalent cation in some instances). Structural evidence shows that S100 proteins form antiparallel homo- or heterodimers that coordinate binding partner proximity in a calcium-dependent (and sometimes calcium-independent) manner. Although structurally and functionally similar, individual members show restricted tissue distribution, are localized in specific cellular compartments, and display unique protein binding partners, which suggests that each plays a specific role in various signaling pathways. In addition to an intracellular role, some S100 proteins have been shown to act as receptors for extracellular ligands or are secreted and exhibit cytokine-like activities (1-4).

Research studies have shown that S100A4 is overexpressed in highly metastatic cancers, which makes it useful as a marker of tumor progression (5,6) and may serve as a prognostic factor in several cancer types (7-10). S100A4 exerts its function via direct interaction with a number of proteins including P53, P63, nonmuscle myosin IIA, α6β4 integrin, and liprin b1 (11-15). S100A4 is present in the nucleus, cytoplasm and extracellular space. Intracellular and extracellular S100A4 both promote cell migration via interaction with different proteins. Researchers have recently discovered that S100A4 also functions as a neuroprotectant in the peripheral nervous system (16,17).

  1. Heizmann, C.W. et al. (2002) Front Biosci 7, d1356-68.
  2. Donato, R. (2003) Microsc Res Tech 60, 540-51.
  3. Marenholz, I. et al. (2004) Biochem Biophys Res Commun 322, 1111-22.
  4. Santamaria-Kisiel, L. et al. (2006) Biochem J 396, 201-14.
  5. Ismail, N.I. et al. (2008) Cancer Cell Int 8, 12.
  6. Ismail, T.M. et al. (2010) J Biol Chem 285, 914-22.
  7. Rudland, P.S. et al. (2000) Cancer Res 60, 1595-603.
  8. Huang, L.Y. et al. (2011) World J Gastroenterol 17, 69-78.
  9. Wang, L.J. et al. (2012) Appl Immunohistochem Mol Morphol 20, 71-6.
  10. Kang, Y.G. et al. (2012) J Surg Oncol 105, 119-24.
  11. Kriajevska, M.V. et al. (1994) J Biol Chem 269, 19679-82.
  12. Takenaga, K. et al. (1994) J Cell Biol 124, 757-68.
  13. Kriajevska, M. et al. (2002) J Biol Chem 277, 5229-35.
  14. Kim, T.H. et al. (2009) Mol Cancer Res 7, 1605-12.
  15. van Dieck, J. et al. (2010) Oncogene 29, 2024-35.
  16. Dmytriyeva, O. et al. (2012) Nat Commun 3, 1197.
  17. Moldovan, M. et al. (2013) Mol Med 19, 43-53.

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For Research Use Only. Not for Use in Diagnostic Procedures.
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