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S100A8 (E4F8V) Rabbit Monoclonal Antibody #47310

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  • WB
  • IHC

    Product Specifications

    REACTIVITY M
    SENSITIVITY Endogenous
    MW (kDa) 9
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IHC-Immunohistochemistry 
    Species Cross-Reactivity Key:
    • M-Mouse 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    IHC Leica Bond 1:1600 - 1:6400
    Immunohistochemistry (Paraffin) 1:1600 - 1:6400

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    For a carrier-free (BSA and azide free) version of this product see product #70802.

    Protocol

    Specificity / Sensitivity

    S100A8 (E4F8V) Rabbit Monoclonal Antibody recognizes endogenous levels of total S100A8 protein.

    Species Reactivity:

    Mouse

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with mouse S100A8 recombinant protein.

    Background

    S100A8 and S100A9 are calcium-binding proteins that form a noncovalent heterodimer present in monocytes, neutrophils, macrophages, and some epithelial cells (1,2). S100A8 and S100A9 are secreted by a tubulin-dependent mechanism during inflammatory conditions and have antimicrobial and chemotactic functions (3-5). Extracellular S100A8/S100A9 also induces an inflammatory response in endothelial cells, including induction of proinflammatory chemokines and adhesion molecules and increased vascular permeability (6). S100A8/S100A9 induces and recruits myeloid-derived suppressor cells (MDSC) in tumor-bearing mice (7). MDSC produce additional S100A8/S100A9 themselves, resulting in a positive feedback mechanism that sustains MDSC accumulation (7). S100A8/S100A9 is also highly expressed in psoriatic skin, where it directly upregulates transcription of complement protein C3, which contributes to disease (8). In addition, tumor-infiltrating myeloid cells induce expression of S100A8 and S100A9 in cancer cells, which increases invasiveness and metastasis (9).

    Alternate Names

    60B8A; 60B8Ag; AI323541; B8A; B8Ag; Caga; Calgranulin-A; CFA; CFAg; Chemotactic cytokine CP-10; chemotactic S100 protein; CP-1; CP-10; Leukocyte L1 complex light chain; Migration inhibitory factor-related protein 8; MRP-8; Mrp8; p8; Pro-inflammatory S100 cytokine; Protein S100-A8; S100 calcium binding protein A8 (calgranulin A); S100 calcium-binding protein A8; S100a8; S10A8

    For Research Use Only. Not for Use in Diagnostic Procedures.
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