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PhosphoSitePlus® Resource

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Product Includes Quantity Applications Reactivity MW(kDa) Isotype
Phospho-SAPK/JNK (Thr183/Tyr185) (81E11) Rabbit mAb 4668 100 µl
Western Blotting Immunoprecipitation Immunohistochemistry
H M R Dm Sc 46, 54 Rabbit IgG
SAPK/JNK Antibody 9252 200 µl
Western Blotting
H M R Hm Mk Z B Sc 46, 54 Rabbit 

Product Description

PhosphoPlus® Duets from Cell Signaling Technology (CST) provide a means to assess protein activation status. Each Duet contains an activation-state and total protein antibody to your target of interest. These antibodies have been selected from CST's product offering based upon superior performance in specified applications.


The stress-activated protein kinase/Jun-amino-terminal kinase SAPK/JNK is potently and preferentially activated by a variety of environmental stresses including UV and gamma radiation, ceramides, inflammatory cytokines, and in some instances, growth factors and GPCR agonists (1-6). As with the other MAPKs, the core signaling unit is composed of a MAPKKK, typically MEKK1-MEKK4, or by one of the mixed lineage kinases (MLKs), which phosphorylate and activate MKK4/7. Upon activation, MKKs phosphorylate and activate the SAPK/JNK kinase (2). Stress signals are delivered to this cascade by small GTPases of the Rho family (Rac, Rho, cdc42) (3). Both Rac1 and cdc42 mediate the stimulation of MEKKs and MLKs (3). Alternatively, MKK4/7 can be activated in a GTPase-independent mechanism via stimulation of a germinal center kinase (GCK) family member (4). There are three SAPK/JNK genes each of which undergoes alternative splicing, resulting in numerous isoforms (3). SAPK/JNK, when active as a dimer, can translocate to the nucleus and regulate transcription through its effects on c-Jun, ATF-2, and other transcription factors (3,5).


1.  Davis, R.J. (1999) Biochem Soc Symp 64, 1-12.

2.  Ichijo, H. (1999) Oncogene 18, 6087-93.

3.  Kyriakis, J.M. and Avruch, J. (2001) Physiol Rev 81, 807-69.

4.  Kyriakis, J.M. (1999) J Biol Chem 274, 5259-62.

5.  Leppä, S. and Bohmann, D. (1999) Oncogene 18, 6158-62.

6.  Whitmarsh, A.J. and Davis, R.J. (1998) Trends Biochem Sci 23, 481-5.


Entrez-Gene Id 5599
Swiss-Prot Acc. P45983


For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
PhosphoPlus is a trademark of Cell Signaling Technology, Inc.
U.S. Patent No. 5,675,063.

8206
PhosphoPlus® SAPK/JNK (Thr183/Tyr185) Antibody Duet