Cat. # | Size | Qty. | Price |
---|---|---|---|
74471S | 100 µl |
|
REACTIVITY | Vir |
SENSITIVITY | Transfected Only |
MW (kDa) | 20-30 |
SOURCE | Rabbit |
Product Information
Application | Dilution |
---|---|
Western Blotting | 1:1000 |
For western blots, incubate membrane with diluted primary antibody in 5% w/v nonfat dry milk, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
NOTE: Please refer to primary antibody product webpage for recommended antibody dilution.
NOTE: Prepare solutions with reverse osmosis deionized (RODI) or equivalent grade water.
Load 20 µl onto SDS-PAGE gel (10 cm x 10 cm).
NOTE: Loading of prestained molecular weight markers (#59329, 10 µl/lane) to verify electrotransfer and biotinylated protein ladder (#7727, 10 µl/lane) to determine molecular weights are recommended.
NOTE: Volumes are for 10 cm x 10 cm (100 cm2) of membrane; for different sized membranes, adjust volumes accordingly.
* Avoid repeated exposure to skin.
posted June 2005
revised June 2020
Protocol Id: 263
Virus
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly211 of SARS-CoV-1 membrane protein. Antibodies are purified by peptide affinity chromatography.
The cause of the SARS epidemic in 2003 was a novel pathogenic coronavirus, originally termed SARS-CoV (severe acute respiratory syndrome coronavirus) (1). Following the 2019 emergence of SARS-CoV-2 and the COVID-19 pandemic, SARS-CoV is now often referred to as SARS-CoV-1. SARS-CoV-1 is a member of the Coronaviridae family of single-stranded RNA viruses (2). The genome of SARS-CoV-1 is relatively large among viruses (~30 kDa); it encodes four key structural proteins (spike, envelope, membrane, and nucleocapsid) that make up the virion particle, in addition to a variety of smaller open reading frames (ORFs) that encode the accessory proteins required for viral replication (3). The SARS-CoV-1 membrane protein (M) is the most abundant protein in the viral envelope; in addition to its structural role, it plays an important role in virus-host interactions and the viral lifecycle (4). Notably, studies have shown that membrane proteins from both SARS-CoV-1 and SARS-CoV-2 may induce apoptosis in host cells, suggesting one mechanism by which SARS coronaviruses cause tissue damage upon infection (5,6).
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