Render Target: STATIC
Render Timestamp: 2024-10-09T09:37:50.484Z
Commit: f04ddd7fea9fb3592f59f61482fcb94610d25cbe
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PDP - Template Name: Polyclonal Antibody
PDP - Template ID: *******59c6464
  1. Products /
  2. Primary Antibodies /
  3. SARS-CoV-2 EndoRNAse Antibody

SARS-CoV-2 EndoRNAse Antibody #62823

Filter:
  • WB

    Supporting Data

    REACTIVITY Vir
    SENSITIVITY Transfected Only
    MW (kDa) 45
    SOURCE Rabbit
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • Vir-Virus 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    SARS-CoV-2 EndoRNAse Antibody recognizes transfected levels of total SARS-CoV-2 endoRNAse protein.

    Species Reactivity:

    Virus

    Source / Purification

    Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of SARS-CoV-2 endoRNAse protein. Antibodies are purified by peptide affinity chromatography.

    Background

    The cause of the COVID-19 pandemic is a novel and highly pathogenic coronavirus, termed SARS-CoV-2 (severe acute respiratory syndrome coronavirus-2). SARS-CoV-2 is a member of the Coronaviridae family of viruses (1). The genome of SARS-CoV-2 is relatively large and encodes up to 29 open reading frames (ORFs). These include ORF1a and ORF1b (further processed into 16 non-structural proteins), 9 accessory proteins, and 4 canonical structural proteins: spike (S), envelope (E), membrane (M), and nucleocapsid (N) (2). SARS-CoV-2 Endonuclease, also called Nsp15, is translated from ORF1b. It is a uridine specific endoribonuclease that is thought to specifically cleave polyuridine RNA sequence at the 5’ end of the template negative strand. Since polyuridine RNA can trigger the PAMP response, this action of the nuclease may block interferon production (3). It is also proposed to prevent accumulation of dsRNA intermediates and the formation of stress granules which are cell anti-viral responses (4). Together, these activities may prevent activation of host cell immune responses.

    Database Links

    UniProt ID:


    Entrez-Gene Id: 43740578


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    For Research Use Only. Not For Use In Diagnostic Procedures.
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