Render Target: STATIC
Render Timestamp: 2025-03-21T10:42:29.490Z
Commit: 779953b12a5930618aae6aca7c87fb286faeb1d7
XML generation date: 2025-03-07 13:16:17.365
Product last modified at: 2025-01-01T09:03:01.212Z
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PDP - Template Name: Polyclonal Antibody
PDP - Template ID: *******59c6464

SARS-CoV-2 Nsp1 Antibody #57896

Filter:
  • WB

    Supporting Data

    REACTIVITY Vir
    SENSITIVITY Endogenous
    MW (kDa) 20-25
    SOURCE Rabbit
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • Vir-Virus 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    SARS-CoV-2 Nsp1 Antibody recognizes endogenous levels of total SARS-CoV-2 Nsp1 protein in infected cells.

    Species Reactivity:

    Virus

    Source / Purification

    Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala76 of SARS-CoV-2 Nsp1 protein. Antibodies are purified by peptide affinity chromatography.

    Background

    The cause of the COVID-19 pandemic is a novel and highly pathogenic coronavirus, termed SARS-CoV-2 (severe acute respiratory syndrome coronavirus-2). SARS-CoV-2 is a member of the Coronaviridae family of viruses (1). The genome of SARS-CoV-2 is relatively large and encodes up to 29 open reading frames (ORFs). These include ORF1a and ORF1b (further processed into 16 non-structural proteins), 9 accessory proteins, and 4 canonical structural proteins: spike (S), envelope (E), membrane (M), and nucleocapsid (N) (2). Nsp1 is the first protein to be translated from the viral RNA by the eukaryotic translational machinery. It has previously been shown that Nsp1 from SARS-CoV inhibits the host response to viral RNA by inhibiting host cell translation including mRNA encoding Type 1 interferon (3). The ability of SARS-CoV-2 Nsp1 has also been shown to exert inhibition of host cell translation, specifically by binding to the 40S ribosomal subunit, and competing out entry of M7G capped eukaryotic mRNA to the entry channel of the pre-initiation translation complex (4,5).
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