Cell Signaling Technology Logo
View Featured Offers >>
1% for the planet logo
  1. Products /
  2. Primary Antibodies /
  3. SARS-CoV-2 ORF3a Antibody

SARS-CoV-2 ORF3a Antibody #34340

Filter:
  • WB
  • IP

    Supporting Data

    REACTIVITY Vir
    SENSITIVITY Endogenous
    MW (kDa) 25-31
    SOURCE Rabbit
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • Vir-Virus 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:50

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    SARS-CoV-2 ORF3a Antibody recognizes endogenous levels of total SARS-CoV-2 ORF3a protein.

    Species Reactivity:

    Virus

    Source / Purification

    Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ile232 of SARS-CoV-2 ORF3a protein. Antibodies are purified by protein A and peptide affinity chromatography.

    Background

    SARS-CoV-2 ORF3a is the largest of the SARS-CoV-2 accessory proteins containing three amino-terminal transmembrane domains localized to the plasma membrane and perinuclear regions including the golgi apparatus (1). It contains several functional domains including a TRAF3 binding motif, a caveolin-binding motif, a tyrosine-sorting motif, and a diacidic motif (2). SARS-CoV ORF3a was shown to regulate apoptotic and necrotic cell death and inhibit late stages of autophagy by inhibiting fusion of the autophagosome and lysosome (3-5). SARS-CoV-2 ORF3a was also found to inhibit late stages of autophagy by inhibiting fusion of the autophagosome and lysosome through association with components of the homotypic fusion and protein sorting HOPS complex (6-8). It is thought that SARS-CoV-2 can form ion channels that regulates viral release (9). In addition, it can regulate several aspects of innate immune responses, including TRAF3 signaling, activation of NF-κB, and production of inflammatory cytokines (10,11).
    α

    Database Links

    UniProt ID: P0DTC3


    Entrez-Gene Id: 43740569


    Limited Uses

    Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

    Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

    For Research Use Only. Not For Use In Diagnostic Procedures.
    Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
    XP is a registered trademark of Cell Signaling Technology, Inc.
    All other trademarks are the property of their respective owners. Visit our Trademark Information page.