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Semaphorin-7A (F2L1S) Rabbit Monoclonal Antibody

Semaphorin-7A (F2L1S) Rabbit Monoclonal Antibody #45588

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Product Specifications

REACTIVITY	H M R
SENSITIVITY	Endogenous
MW (kDa)	80
Source/Isotype	Rabbit IgG

Application Key:

WB-Western Blotting

Species Cross-Reactivity Key:

H-Human
M-Mouse
R-Rat

Product Information

Product Usage Information

Application	Dilution
Western Blotting	1:1000

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

Semaphorin-7A (F2L1S) Rabbit Monoclonal Antibody recognizes endogenous levels of total semaphorin-7A protein.

Species Reactivity:

Human, Mouse, Rat

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro400 of human Semaphorin-7A protein.

Background

Semaphorin-7A is a GPI-anchored membrane protein that plays important roles in neuronal development and immune responses via integrin-mediated signaling. It promotes axon growth by activating the mitogen-activated protein kinase (MAPK) pathway through β1 integrin receptors. When expressed on activated T cells, it promotes the production of proinflammatory cytokines by macrophages through the α1β1 integrin receptor (1). Semaphorin-7A may also play a role in resolving inflammation through its regulation of macrophage chemotaxis and chemokinesis, and by promoting macrophage differentiation and polarization toward an anti-inflammatory phenotype (2). Neuroinflammation is a detrimental component of neurodegenerative diseases, such as Parkinson’s disease (PD) and multiple sclerosis (MS). The role that semaphorin-7A plays in mediating inflammatory responses in microglia in the brain suggests that it may be a therapeutic target for these diseases. In a cell model of neuroinflammation, semaphorin-7A was shown to promote cell viability and survival through its mediation of MAPK and peroxisome proliferator-activated receptor γ (PPARγ) pathways (3).

Alternate Names

CD108; CDw108; H-SEMA-K1; H-Sema-L; JMH; JMH blood group antigen; John Milton Hagen blood group H-Sema K1; John-Milton-Hargen human blood group Ag; MGC126692; MGC126696; SEM7A; sema domain, immunoglobulin domain (Ig), and GPI membrane anchor, (semaphorin) 7A (JMH blood group); sema domain, immunoglobulin domain (Ig), and GPI membrane anchor, 7A; Sema K1; Sema L; SEMA7A; SEMAK1; SEMAL; semaphorin 7A (John Milton Hagen blood group); semaphorin 7A, GPI membrane anchor (John Milton Hagen blood group); semaphorin K1; semaphorin L; Semaphorin-7A; Semaphorin-K1; Semaphorin-L

Database Links

UniProt ID: O75326

Entrez-Gene Id: 8482

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