Render Target: STATIC
Render Timestamp: 2024-10-11T10:08:29.874Z
Commit: 56767fe525c928647c8401233a175d0d607d385d
XML generation date: 2024-08-01 15:28:33.813
Product last modified at: 2024-09-26T11:45:20.357Z
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PDP - Template Name: Polyclonal Antibody
PDP - Template ID: *******59c6464

SF3A3 Antibody #47147

Filter:
  • WB
  • IP

    Supporting Data

    REACTIVITY H M R Mk
    SENSITIVITY Endogenous
    MW (kDa) 62
    SOURCE Rabbit
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 
    • Mk-Monkey 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:50

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    SF3A3 Antibody recognizes endogenous levels of total SF3A3 protein.

    Species Reactivity:

    Human, Mouse, Rat, Monkey

    Source / Purification

    Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu495 of human SF3A3 protein. Antibodies are purified by peptide affinity chromatography.

    Background

    The mammalian splicing factor SF3a contains three primary subunits: SF3A1 (SF3a120), SF3A2 (SF3a66), and SF3A3 (SF3a60) which, along with SF3b, activate the U2 snRNP to form the pre-mRNA splicing complex (1,2). SF3A2 and SF3A3 both interact with SF3A1, but not with each other, and SF3A3 plays a major role in recruiting SF3A1 to the complex (3). Each protein in the heterotrimer has been shown to be essential for mRNA splicing and cell viability (4). Notably, SF3a plays a role in growth factor signaling as SF3A1 can bind to ERalpha when phosphorylated at Ser118 to affect downstream splicing (5). SF3a also plays a role in the innate immune response, as it promotes inflammation by splicing the TLR adaptor Myd88. Furthermore, SF3a regulates a number of genes involved in innate immune signaling pathways in macrophages versus other pathways (6,7).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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