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2287
Shh Antibody

Shh Antibody #2287

This product is discontinued

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# Product Name Application Reactivity
  • WB
H R Z
Storage:

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Shh antibody detects both the precursor and mature forms of transfected Shh.

Species predicted to react based on 100% sequence homology:

Human

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding His35 of human Shh. Antibodies are purified by protein A and peptide affinity chromatography.

Hedgehog proteins (Hh) are secreted signaling proteins that play many roles during animal development. Aberrant Hh signaling activity can be associated with numerous birth defects and uncontrolled Hh pathway activation is linked to the development of several types of cancers (1-2). The three identified vertebrate Hh genes are Sonic (Shh), Indian (Ihh) and Desert (Dhh), all of which have distinct as well as overlapping roles (3-5). Hh proteins are synthesized as 45 kDa precursors that undergo auto-cleavage to generate a 19 kDa amino-terminal peptide (Hh-N) and a carboxy-terminal peptide (Hh-C). The amino-terminal peptide becomes covalently attached to a cholesterol molecule at its carboxy terminus and acetylated at its amino terminus. This doubly modified Hh-N peptide is released from cells and responsible for all known Hedgehog signaling activity (6).

  1. Ingham, P.W. and McMahon, A.P. (2001) Genes Dev 15, 3059-87.
  2. McMahon, A.P. et al. (2003) Curr Top Dev Biol 53, 1-114.
  3. Zhang, X.M. et al. (2001) Cell 106, 781-92.
  4. Adolphe, C. et al. (2004) Development 131, 5009-19.
  5. Pathi, S. et al. (2001) Mech Dev 106, 107-17.
  6. Bijlsma, M.F. et al. (2004) Bioessays 26, 387-394.
Entrez-Gene Id
6469
Swiss-Prot Acc.
Q15465
For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

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