Render Target: STATIC
Render Timestamp:
3/25/2025, 6:29:41 AM EDT
3/25/2025, 10:29:41 AM UTC
Commit: 8d93f7ebe45006d66c127727d817fc3f57c4fe9a
XML generation date: 2025-03-07 13:09:28.421
Product last modified at: 2025-01-01T09:04:20.626Z
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PDP - Template Name: Polyclonal Antibody
PDP - Template ID: *******59c6464

SLK Antibody #41255

Filter:
  • WB
  • IP

    Supporting Data

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 220
    SOURCE Rabbit
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:50

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    SLK antibody recognizes endogenous levels of total SLK protein.

    Species Reactivity:

    Human

    Source / Purification

    Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Glu431 of human SLK protein.

    Background

    SLK (Ste20-like Kinase) is a member of the germinal center kinase (GCK) family of proteins. SLK has a kinase domain located at the N terminus (1). The autophosphorylation of SLK at Thr183 and Ser189 is required for the upregulation of SLK kinase activity (1, 2). The protein also has a caspase cleavage site DXXD and a SH3 binding site PXXP located in the middle part of its sequence, and a regulatory C terminal coiled-coil domain for homodimerization and adaptor binding (1-4). SLK plays important roles in development, tissue regeneration and cancer cell migration by regulating several signaling pathways (5-7). SLK phosphorylates and activates ASK1 to induce downstream p38 phosphorylation and apoptosis (8,9). During cell cycle, SLK phosphorylates Polo-like kinase (PLK) at Thr210 to promote G2/M transition (10,11). SLK also promotes cell division by direct phosphorylation of ERMs and dynactin to activate microtubule reorganization and spindle orientation (12, 13). During focal adhesion and cell migration process, SLK is activated and colocalized to the focal adhesion complex where it promotes complex turnover by phosphorylating paxillin at Ser250 (14, 15).
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