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Primary Antibodies /
Smac/Diablo Antibody

Smac/Diablo Antibody #2952

Inquiry Info. # 2952

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Product Specifications

REACTIVITY
SENSITIVITY
MW (kDa)	26
SOURCE	Rabbit

Product Information

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Specificity / Sensitivity

Smac/Diablo Antibody detects total levels of endogenous Smac/Diablo protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser 117 of human Smac. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Smac/Diablo is a 21 kDa mammalian mitochondrial protein that functions as a regulatory component during apoptosis (1,2). Upon mitochondrial stress, Smac/Diablo is released from mitochondria and competes with caspases for binding of IAPs (inhibitor of apoptosis proteins) (1,2). The interaction of Smac/Diablo with IAPs relieves the inhibitory effect of the IAPs on caspases (3,4). This interaction involves mainly the amino-terminal residues of Smac/Diablo with the BIR3 region of XIAP, supplemented with several other hydrophobic interactions between the helical structures of Smac/Diablo and other areas of BIR3 (5,6).

Alternate Names

0610041G12Rik; DBLOH; DFNA64; DIABLO; diablo homolog (Drosophila); Diablo homolog, mitochondrial; Diablo IAP-binding mitochondrial protein; diablo-like protein; DIABLO-S; Direct IAP-binding protein with low pI; FLJ10537; FLJ25049; mitochondrial Smac protein; Second mitochondria-derived activator of caspase; SMAC; SMAC3

Database Links

UniProt ID: Q9NR28

Entrez-Gene Id: 56616

Limited Uses

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For Research Use Only. Not for Use in Diagnostic Procedures.

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