Smurf1 Antibody #2174
- WB
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 81 |
| SOURCE | Rabbit |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Protocol
Specificity / Sensitivity
Species Reactivity:
Source / Purification
Background
Smurf1, a member of the HECT family of E3 ubiquitin ligases, selectively interacts with BMP pathway Smad effectors, leading to Smad protein ubiquitination and degradation (6). In addition, Smurf1 interacts with the inhibitor Smad, Smad7, the bone-specific transcription factor Runx2/Cbfa1, RhoA and MEKK2 (7-10). Smurf1 negatively regulates osteoblast differentiation and bone formation in vivo (10,11). A related protein, Smurf2, acts more promiscuously, interacting with both BMP and TGF-β pathway Smad proteins (12).
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- Hoodless, P.A. et al. (1996) Cell 85, 489-500.
- Klemm, J.D. et al. (1998) Annu Rev Immunol 16, 569-92.
- Kretzschmar, M. et al. (1997) Genes Dev 11, 984-95.
- Whitman, M. (1998) Genes Dev 12, 2445-62.
- Sapkota, G. et al. (2007) Mol Cell 25, 441-54.
- Alarcón, C. et al. (2009) Cell 139, 757-69.
- Zhu, H. et al. (1999) Nature 400, 687-693.
- Ebisawa, T. et al. (2001) J. Biol. Chem. 276, 12477-12480.
- Zhao, M. et al. (2003) J. Biol. Chem. 278, 27939-27944.
- Wang, H.R. et al. (2003) Science 302, 1775-1779.
- Yamashita, M. et al. (2005) Cell 121, 101-113.
- Zhao, M. et al. (2004) J. Biol. Chem. 279, 12854-12859.
- Zhang, Y. et al. (2001) Proc. Natl. Acad. Sci. U S A 98, 974-979.
Alternate Names
E3ligase
Limited Uses
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