Render Target: STATIC
Render Timestamp: 2024-12-02T10:59:25.299Z
Commit: cd2fae6ca3f811b1ddb1df24ac291ed56d5d501b
XML generation date: 2024-09-30 01:55:42.754
Product last modified at: 2024-11-26T15:45:09.317Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

SOCS3 (D6E1T) Rabbit mAb #52113

Filter:
  • WB

    Supporting Data

    REACTIVITY H M
    SENSITIVITY Endogenous
    MW (kDa) 28
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    SOCS3 (D6E1T) Rabbit mAb recognizes endogenous levels of total SOCS3 protein.

    Species Reactivity:

    Human, Mouse

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with recombinant protein specific to human SOCS3 protein.

    Background

    The suppressor of cytokine signaling (SOCS) family members are negative regulators of cytokine signal transduction that inhibit the Jak/Stat pathway (1-3). The SOCS family consists of at least 8 members including the originally identified cytokine-inducible SH2-containing protein (CIS1), as well as SOCS1-7. Each SOCS family member contains a central SH2 domain and a conserved carboxy-terminal motif designated as the SOCS box. These proteins are important regulators of cytokine signaling, proliferation, differentiation, and immune responses.
    Low levels of SOCS3 are observed in lung, spleen, and thymus and, like other SOCS family members, its expression is rapidly induced by a number of factors including interleukins, EPO, IFN-γ, CSF, and TNF-α (4). SOCS3 uses its SH2 domain to bind activated Jaks and their cognate receptors to provide negative feedback inhibition. In addition to the initially described inducers of SOCS3 expression, subsequent studies have described SOCS3-mediated negative feedback inhibition for leptin (5), GH (6), chemokine receptors (7), insulin (8), and certain pathogens (9,10). SOCS3 deletion results in embryonic lethality with placental insufficiency (11). SOCS3 signaling has been linked pathologically to allergic responses (12), inflammatory disease (13), endotoxic shock (14), wound repair (15), and obesity (16,17).
    1. Alexander, W.S. et al. (1999) J Leukoc Biol 66, 588-92.
    2. Chen, X.P. et al. (2000) Immunity 13, 287-90.
    3. Hilton, D.J. et al. (1998) Proc Natl Acad Sci USA 95, 114-9.
    4. Starr, R. et al. (1997) Nature 387, 917-21.
    5. Bjørbaek, C. et al. (1998) Mol Cell 1, 619-25.
    6. Adams, T.E. et al. (1998) J Biol Chem 273, 1285-7.
    7. Soriano, S.F. et al. (2002) J Exp Med 196, 311-21.
    8. Emanuelli, B. et al. (2000) J Biol Chem 275, 15985-91.
    9. Stoiber, D. et al. (1999) J Immunol 163, 2640-7.
    10. Stoiber, D. et al. (2001) J Immunol 166, 466-72.
    11. Roberts, A.W. et al. (2001) Proc Natl Acad Sci U S A 98, 9324-9.
    12. Seki, Y. et al. (2003) Nat Med 9, 1047-54.
    13. Shouda, T. et al. (2001) J Clin Invest 108, 1781-8.
    14. Fang, M. et al. (2005) Cell Mol Immunol 2, 373-7.
    15. Goren, I. et al. (2006) J Invest Dermatol 126, 477-85.
    16. Mori, H. et al. (2004) Nat Med 10, 739-43.
    17. Howard, J.K. et al. (2004) Nat Med 10, 734-8.
    For Research Use Only. Not For Use In Diagnostic Procedures.
    Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
    XP is a registered trademark of Cell Signaling Technology, Inc.
    KARPAS cell line source: Dr. Abraham Karpas at the University of Cambridge.
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