Sox10 (D5V9L) Rabbit mAb #89356
- WB
- IP
- IF
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 62 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
- IF-Immunofluorescence
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Simple Western™ | 1:50 - 1:250 |
| Immunoprecipitation | 1:100 |
| Immunofluorescence (Immunocytochemistry) | 1:1600 |
Storage
Protocol
Specificity / Sensitivity
Sox10 (D5V9L) Rabbit mAb recognizes endogenous levels of total Sox10 protein. This antibody also recognizes presumptive sumoylated forms of Sox10 protein.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu249 of human Sox10 protein.
Background
SRY-box 10 (Sox10) is a member of the SOX (SRY-related HMG-box) family of transcription factors involved in the regulation of embryonic development and the determination of cell fate. Sox10 is an important regulator of neural crest and peripheral nervous system development (1-3). Mutations in the SOX10 gene are associated with a group of auditory-pigmentary developmental disorders, including Waardenburg and Waardenburg-Shah syndromes (3,4). Research studies suggest an oncogenic role for Sox10 in various tumor types, such as hepatocellular carcinoma and melanoma (5,6).
- Hong, C.S. and Saint-Jeannet, J.P. (2005) Semin Cell Dev Biol 16, 694-703.
- Britsch, S. et al. (2001) Genes Dev 15, 66-78.
- Bondurand, N. and Sham, M.H. (2013) Dev Biol 382, 330-43.
- Mollaaghababa, R. and Pavan, W.J. (2003) Oncogene 22, 3024-34.
- Zhou, D. et al. (2014) Tumour Biol 35, 9935-40.
- Graf, S.A. et al. (2014) J Invest Dermatol 134, 2212-20.
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