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  2. Primary Antibodies /
  3. SPARCL1 (F3V2I) Mouse mAb

SPARCL1 (F3V2I) Mouse mAb #26648

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  • IP
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    Supporting Data

    REACTIVITY M R
    SENSITIVITY Endogenous
    MW (kDa) 140
    Source/Isotype Mouse IgG1 kappa
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    • IF-Immunofluorescence 
    Species Cross-Reactivity Key:
    • M-Mouse 
    • R-Rat 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:50
    Immunofluorescence (Frozen) 1:100 - 1:400

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    SPARCL1 (F3V2I) Mouse mAb recognizes endogenous levels of total SPARCL1 protein.

    Species Reactivity:

    Mouse, Rat

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val136 of mouse SPARCL1 protein.

    Background

    SPARC-like protein 1 (SPARCL1) is a cysteine-rich protein secreted into the extracellular matrix that belongs to the SPARC protein family. SPARC proteins (secreted protein acidic and rich in cysteine) are secreted glycoproteins involved in tissue renewal and remodeling, and embryonic development (1). SPARC proteins are highly upregulated in melanoma and other tumor types, but their contributing functions are not yet understood (2). However, the role SPARC proteins play in regulation of extracellular matrix interactions is likely to contribute to melanoma development and metastasis. SPARCL1 is likely to play a similar role in extracellular matrix remodeling in the vasculature and the brain. For example, SPARCL1 is upregulated in vascular endothelial cells in response to injury and infection, both remodeling the extracellular matrix and promoting pro-inflammatory macrophage activation (3). SPARCL1 is also expressed in glial cells in the brain, specifically radial glial cells during brain development and in mature astrocytes where it may play a role in the formation of glutamatergic synapses (4,5).

    Database Links

    UniProt ID: P70663


    Entrez-Gene Id: 13602


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