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SRRM2 (F3J1Z) Rabbit Monoclonal Antibody #19700

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  • IP
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    Product Specifications

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 200-300
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    • IF-Immunofluorescence 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:50
    Immunofluorescence (Immunocytochemistry) 1:2000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    SRRM2 (F3J1Z) Rabbit Monoclonal Antibody recognizes endogenous levels of total SRRM2 protein. This antibody detects a 42 kDa protein of unknown identity in some cell lines.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the central region of human SRRM2 protein.

    Background

    Nuclear speckles (NS) are distinct biomolecular condensates consisting of nuclear proteins and RNA molecules. Although their exact biological function is not fully understood, NS are believed to function as hubs for mRNA splicing and potentially transcription (1). The splicing factors serine/arginine repetitive matrix protein 2 (SRRM2) and SON are necessary for NS formation (2). SRRM2 and SON undergo phase separation and function as scaffolding proteins, recruiting additional splicing factors and promoting NS assembly (3). In Alzheimer’s disease (AD) and other tauopathies, SRRM2 can become mislocalized from NS to pathological tau aggregates in the cytoplasm, resulting in aberrant splicing (4,5). Loss-of-function (LoF) mutations in SRRM2 have also been associated with neurodevelopmental delay and an increased risk of developing papillary thyroid carcinoma (PTC) (6,7).

    Alternate Names

    300 kDa nuclear matrix antigen; 300-KD; Cwc21; CWF21; DKFZp667L0421; DKFZp686O15166; FLJ21926; FLJ22250; HSPC075; KIAA0324; MGC40295; RNA binding protein; Ser/Arg-related nuclear matrix protein of 300 kDa; serine/arginine repetitive matrix 2; Serine/arginine repetitive matrix protein 2; Serine/arginine-rich splicing factor-related nuclear matrix protein of 300 kDa; Splicing coactivator subunit SRm300; SR-related nuclear matrix protein of 300 kDa; SRL300; SRM300; SRRM2; Tax-responsive enhancer element-binding protein 803; TaxREB803; testicular secretory protein Li 53

    For Research Use Only. Not for Use in Diagnostic Procedures.
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