Render Target: STATIC
Render Timestamp: 2024-12-02T11:14:39.278Z
Commit: cd2fae6ca3f811b1ddb1df24ac291ed56d5d501b
XML generation date: 2024-08-01 15:28:33.892
Product last modified at: 2024-08-19T12:15:08.605Z
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PDP - Template Name: Polyclonal Antibody
PDP - Template ID: *******59c6464

SRSF3 Antibody #51039

Filter:
  • WB

    Supporting Data

    REACTIVITY H M R Mk
    SENSITIVITY Endogenous
    MW (kDa) 20, 22
    SOURCE Rabbit
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 
    • Mk-Monkey 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    SRSF3 Antibody recognizes endogenous levels of total SRSF3 protein.

    Species Reactivity:

    Human, Mouse, Rat, Monkey

    Source / Purification

    Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly83 of human SRSF3 protein. Antibodies are purified by peptide affinity chromatography.

    Background

    Serine/arginine-rich splicing factor 3 (SRSF3), also known as SRP20, is a member of the highly conserved family of SR proteins responsible for splicing regulation (1,2). SRSF3 promotes exon inclusion by enhancing the recognition of weak splice acceptor sites, a function that also regulates its own mRNA by including exon 4. This function is antagonized by another SR family member, SRSF1 (3). SRSF3 can be shuttled in and out of the cytoplasm, where it plays a role in mRNA export along with SRSF7 (3,4). SRSF3 and SRSF7 have been shown to exert opposing effects on 3’UTR length by recruiting various proteins upstream of polyadenylation sites (5,6). SRSF3 has been shown to be regulated by an epitranscriptomic mechanism, as the m6a-binding factor YTHDC1 recruits SRSF3 to promote exon inclusion, while blocking the binding of SRSF10 (7). SRSF3 has been identified as an oncogene, which through its autoregulation and alternative splicing of other proteins, can drive many different cancer types (8-12).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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