Western blot analysis of extracts from COS-7 cells, mock transfected (-) or transfected with a construct expressing human SSTR1 (hSSTR1; +), using SSTR1 Antibody. Multiple bands are thought to be GPCR dimers and/or oligomers (Rozenfeld, R. and Devi, L.A. (2010) Handbook of Cell Signaling, 2nd edition, 185-94). Smearing is thought to be due to the addition of N-linked glycosylations at specific NXS/T motifs (Dong, C. et al. (2007) Biochem Biophys Acta 1768, 853-70).Learn more about how we get our images.
Western blot analysis of extract from PC-12 cells using SSTR1 Antibody.Learn more about how we get our images.
For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
NOTE: Please refer to primary antibody datasheet or product webpage for recommended antibody dilution.
From sample preparation to detection, the reagents you need for your Western Blot are now in one convenient kit: #12957 Western Blotting Application Solutions Kit
NOTE: Prepare solutions with reverse osmosis deionized (RODI) or equivalent grade water.
Load 20 µl onto SDS-PAGE gel (10 cm x 10 cm).
NOTE: Volumes are for 10 cm x 10 cm (100 cm2) of membrane; for different sized membranes, adjust volumes accordingly.
* Avoid repeated exposure to skin.
posted June 2005
revised November 2013
Reprobing of an existing membrane is a convenient means to immunoblot for multiple proteins independently when only a limited amount of sample is available. It should be noted that for the best possible results a fresh blot is always recommended. Reprobing can be a valuable method but with each reprobing of a blot there is potential for increased background signal. Additionally, it is recommended that you verify the removal of the first antibody complex prior to reprobing so that signal attributed to binding of the new antibody is not leftover signal from the first immunoblotting experiment. This can be done by re-exposing the blot to ECL reagents and making sure there is no signal prior to adding the next primary antibody.
NOTE: Prepare solutions with reverse osmosis deionized (RODI) or equivalently purified water.
posted June 2005
revised October 2016
Protocol Id: 10
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
SSTR1 Antibody recognizes endogenous levels of total SSTR1 protein.
Human, Mouse, Monkey
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val365 of human SSTR1 protein. Antibodies are purified by protein A and peptide affinity chromatography.
Somatostatin receptors are part of the super family of G protein-coupled receptors. Five genes encoding six different somatostatin receptor subtypes (SSTR1, SSTR2A, SSTR2B, SSTR3, SSTR4, and SSTR5) have been cloned (1). Somatostatin receptors are activated by somatostatin, a neuropeptide that acts as a neurotransmitter in the brain that regulates hormone secretion from endocrine tissues (2). Somatostatin receptors are found to be highly expressed on human neuroendocrine tumors (3).
mRNA expression data and immunohistochemical studies indicate high expression of SSTR1 in pituitary and gastroenteropancreatic tumor, renal, colorectal and breast cancer, meningioma, glioma neuroblastoma, and pheochromocytoma (3).
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|11830S||100 µl (10 western blots)||$ 255.0|